Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy.
Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy.
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DOI：
10.1016/j.jaci.2008.12.1128
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发表时间：
2009-04
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耐受热变性牛奶（HM）的牛奶过敏儿童的反应不太严重，并且比那些对HM有反应的儿童更早地摆脱这种状况，这可能与IgE依赖性效应细胞功能的差异有关。应用一种新的检测方法来检验HM耐受儿童抑制IgE介导的嗜碱性粒细胞反应的假设。过敏、HM耐受、发育过度或对照受试者通过经口食物激发定义。在存在或不存在自体血清或稀释自体血清的情况下，用一系列牛奶过敏原剂量在体外刺激全血细胞。通过流式细胞术鉴定活化的嗜碱性粒细胞为CD 63 bright CD 123 + CD 203 c + HLA-DR-CD 41 a-。HM耐受性受试者嗜碱性粒细胞对所有剂量的牛奶过敏原刺激的反应性均显著低于HM反应性（过敏）个体的嗜碱性粒细胞。在不存在自体血清的情况下，HM耐受受试者嗜碱性粒细胞在低变应原浓度下反应性显著更强。在较小程度上，自体血清也抑制IL-3和抗IgE诱导的反应，但不抑制fMLP诱导的反应。HM耐受受试者嗜碱性粒细胞的变应原特异性反应性随着用正常混合血清稀释自体血清而增加。牛奶过敏的儿童预后良好，有证据表明外源性抑制过敏原特异性效应细胞反应性。
Milk-allergic children who tolerate heat-denatured milk (HM) have less severe reactions and outgrow the condition earlier than those who react to HM, which may be related to differences in IgE-dependent effector cell function. To apply a novel assay to test the hypothesis that HM-tolerant children have suppressed IgE-mediated basophil responses. Allergic, HM-tolerant, Outgrown or Control subjects were defined by oral food challenges. Whole blood cells were stimulated in vitro with a range of milk allergen doses in the presence or absence of autologous serum or with dilutions of autologous serum. Activated basophils were identified by flow cytometry as CD63bright CD123+ CD203c+ HLA-DR- CD41a-. HM-tolerant subject basophils were significantly less responsive to milk allergen stimulation at all doses than were basophils from HM reactive (Allergic) individuals. In the absence of autologous serum, HM-tolerant subject basophils were significantly more reactive at low allergen concentrations. To a lesser extent, autologous serum also inhibited IL-3- and anti-IgE-induced, but not fMLP-induced responses. The allergen-specific responsiveness of HM-tolerant subject basophils increased with dilution of autologous serum with normal pooled serum. Milk-allergic children with a favorable prognosis have evidence of extrinsically suppressed allergen-specific effector cell reactivity.
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DOI：
10.1084/jem.20060772
发表时间：
2006-07-10
期刊：
The Journal of experimental medicine
影响因子：
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JAMES, JM;SAMPSON, HA
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