Gene Expression Subtype Predicts Nodal Metastasis and Survival in Human Papillomavirus-Negative Head and Neck Cancer.
Gene Expression Subtype Predicts Nodal Metastasis and Survival in Human Papillomavirus-Negative Head and Neck Cancer.
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DOI：
10.1002/lary.27340
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Hayes DN
中科院分区：
文献类型：
--
作者：
Zevallos JP;Mazul AL;Walter V;Hayes DN
头颈癌的基因表达分析揭示了四种分子亚型：基底（BA）、间质（MS）、非典型（AT）和经典（CL）。我们评估口腔（OCSCC）和喉（LSCC）中的基因表达亚型是否可以用于预测淋巴结转移和生存率。2b回顾性队列研究和基因组分析OCSCC和LSCC病例从TCGA头颈癌队列中鉴定。通过预期最大化（RSEM）的RNA-Seq用于量化来自TCGA RNA-seq数据的基因表达水平，并将每个病例分配到四种亚型中的一种。使用描述性统计量描述每个亚型的患者、疾病和治疗特征。采用考克斯回归和Kaplan Meier分析确定与生存率的相关性。OCSCC病例主要由MS和BA亚型组成，而LSCC主要由CL和AT亚型组成。在OCSCC中，MS亚型与淋巴结转移的高风险显著相关。在临床T1- 2N 0 M0 OCSCC的亚组分析中，我们证明MS亚型是隐匿性淋巴结转移的预测因子（RR=3.38，95%CI 1.08-10.69）。在喉鳞状细胞癌中，CL亚型与显著较差的总生存期相关（HR=4.32，95% CI 1.77-10.54，p=0.001）。基因表达分析揭示了HPV（−）头颈癌淋巴结转移和生存的潜在新标志物。未来的研究将继续完善和验证这些标志物，目标是提供分子风险评估，指导治疗和改善患者预后。
Gene expression analyses of head and neck cancer have revealed four molecular subtypes: basal (BA), mesenchymal (MS), atypical (AT), and classical (CL). We evaluate whether gene expression subtypes in oral cavity (OCSCC) and laryngeal (LSCC) can be used to predict nodal metastasis and prognosticate survival. 2b Retrospective cohort study and genomic analysis OCSCC and LSCC cases were identified from the TCGA head and neck cancer cohort. RNA-Seq by Expected Maximization (RSEM) was used to quantify gene expression levels from TCGA RNA-seq data and to assign each case to one of four subtypes. Descriptive statistics were used to describe patient, disease and treatment characteristics in each subtype. Cox regression and Kaplan Meier analyses were used to determine associations with survival. OCSCC cases were comprised primarily of the MS and BA subtypes, while LSCC was comprised primarily of CL and AT subtypes. In OCSCC, the MS subtype was significantly associated with higher risk of nodal metastasis. In a subset analysis of clinically T1-2N0M0 OCSCC, we demonstrate that the MS subtype was predictive of occult nodal metastasis (RR=3.38, 95% CI 1.08–10.69). In LSCC, the CL subtype was associated with significantly worse overall survival (HR=4.32, 95% CI 1.77–10.54, p=0.001). Gene expression analysis reveals potential novel markers of nodal metastasis and survival in HPV (−) head and neck cancer. Future studies will continue to refine and validate these markers, with the goal of providing molecular risk assessments that guide therapy and improve patient outcomes.
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影响因子：
6.2
作者：
Dahlstrom, Kristina R.;Calzada, Gabriel;Hanby, Jennifer D.;Garden, Adam S.;Glisson, Bonnie S.;Li, Guojun;Roberts, Dianna B.;Weber, Randal S.;Sturgis, Erich M.
通讯作者：
Sturgis, Erich M.
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作者：
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11.2
作者：
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通讯作者：
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DOI：
10.1002/hed.23663
发表时间：
2015-05-01
影响因子：
2.9
作者：
Martinez, Victor D.;Vucic, Emily A.;Lam, Wan L.
通讯作者：
Lam, Wan L.