The different effects of over-expressing murine NMDA receptor 2B subunit in the forebrain on conditioned taste aversion
The different effects of over-expressing murine NMDA receptor 2B subunit in the forebrain on conditioned taste aversion
复制标题
前脑过度表达小鼠NMDA受体2B亚基对条件性味觉厌恶的不同影响
DOI：
10.1016/j.brainres.2010.06.004
复制
发表时间：
2010-09
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
据报道，由于使用 NMDA 拮抗剂和激动剂治疗，谷氨酸传输系统和 N-甲基-d-天冬氨酸受体 (NMDA-R)，特别是其 2B 亚基 (NR2B) 可能与味觉记忆相关。为了进一步研究NR2B亚基在味觉记忆中的作用，我们应用了四种不同的味觉厌恶任务来观察转基因小鼠模型的行为，其中NR2B亚基在前脑中特异性过度表达。我们发现，在短期和长期条件性味觉厌恶（CTA）实验中，前脑表达 NR2B 转基因（Tg）的小鼠在训练后 2 天表现出显着增强的 CTA。然而，训练后第 30 天，Tg 小鼠和野生型 (Wt) 小鼠的厌恶记忆水平相同。在快速和慢速灭绝实验中，Tg 小鼠在大多数灭绝试验中都比对照小鼠保持了更高的 CTA 记忆。第三个实验涉及测试对熟悉味道的记忆，证明 NR2B 增强对 CTA 的潜在抑制 (LI) 没有好处。此外，最后一个实验（双味 LI）显示，当 Tg 小鼠接触新口味和熟悉口味时，CTA 增强受到抑制。这些数据表明前脑 NR2B 过度表达对味觉学习和记忆有不同的影响。转基因动物只对新奇的味道敏感，但对熟悉的味道不敏感，并且NR2B的上调仅在短时间内导致CTA功能增强。
The glutamate transmission system and the N-methyl-d-aspartate receptor (NMDA-R), in particular its 2B subunit (NR2B), have been reported to be possibly related to taste memory as a result of treatment with NMDA antagonists and agonists. In order to further study the role of the NR2B subunit in gustation memory, we applied four different taste aversive tasks to observe the behavior of a transgenic mice model in which the NR2B subunit was specifically over-expressed in the forebrain. We found that in both short- and long-term conditioned taste aversion (CTA) experiments, mice with forebrain expression of the NR2B transgene (Tg) showed significantly enhanced CTA 2days after training. However, both the Tg and the wild-type (Wt) mice shared the same level of aversive memory on the 30th day after training. In both fast and slow extinction experiments, Tg mice maintained a higher CTA memory than that of control mice in most extinction trials. The third experiment, which involved testing the memory for familiar taste, demonstrated that NR2B augmentation had no benefit on the latent inhibition (LI) of CTA. In addition, the last experiment (two-taste LI) showed a suppression of enhanced CTA in Tg mice when the mice were exposed to both novel and familiar tastes. These data suggested that forebrain NR2B over-expression had different effects on gustatory learning and memory. The transgenic animals were only sensitive to novel but not familiar tastes, and up-regulation of NR2B resulted in enhanced CTA function for only a short period of time.
登录
查看更多内容
DOI：
10.3758/bf03331929
发表时间：
1997-09
期刊：
Psychobiology
影响因子：
--
作者：
C. Land;D. Riccio
通讯作者：
C. Land;D. Riccio
影响因子：
1.9
作者：
D. Purves;C. Bonardi;G. Hall
通讯作者：
D. Purves;C. Bonardi;G. Hall
影响因子：
2.9
作者：
Takashi Yamamoto;Y. Fujimoto;T. Shimura;N. Sakai
通讯作者：
Takashi Yamamoto;Y. Fujimoto;T. Shimura;N. Sakai
影响因子：
2
作者：
M. Stone;Brandon S. Grimes;D. Katz
通讯作者：
M. Stone;Brandon S. Grimes;D. Katz
影响因子：
56.9
作者：
J. Garcia;D. J. Kimeldorf;R. A. Koelling
通讯作者：
J. Garcia;D. J. Kimeldorf;R. A. Koelling