VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC.
VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC.
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DOI：
10.1038/s41467-022-32897-7
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发表时间：
2022-09-21
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
Polycomb （PcG）沉默对发育至关重要，但如何指定靶点仍不完全清楚。冷诱导拟南芥开花位点C （FLC）的多梳抑制复合体2 （PRC2）沉默为阐明PcG调控提供了一个很好的系统。DNA结合蛋白VAL1与FLC PcG成核区域的结合是重要的一步。VAL1共同免疫沉淀凋亡和剪接相关蛋白（ASAP）复合物和PRC1。在这里，我们发现ASAP和PRC1是FLC共转录抑制和染色质调控所必需的。ASAP突变体在温暖条件下影响FLC的转录，但在寒冷条件下FLC的沉默率不受影响。低温时，prc1介导的H2Aub积累在FLC成核区增加，但与prc1传递的H3K27me3不同，它不会扩散到整个位点。因此，H2Aub参与了FLC向表观遗传沉默的过渡，促进了H3K27me3的积累和长期的表观遗传记忆。总的来说，我们的工作强调了VAL1作为FLC表观遗传沉默所需的组装平台协调活动的重要性。拟南芥FLC的冷诱导沉默需要VAL1与内含子基序结合。本文作者发现，VAL1的两个相互作用的伙伴复合体ASAP和PRC1介导共转录抑制和染色质调节，从而有效地协调FLC沉默的不同步骤。
Polycomb (PcG) silencing is crucial for development, but how targets are specified remains incompletely understood. The cold-induced Polycomb Repressive Complex 2 (PRC2) silencing of Arabidopsis thaliana FLOWERING LOCUS C (FLC) provides an excellent system to elucidate PcG regulation. Association of the DNA binding protein VAL1 to FLC PcG nucleation regionis an important step. VAL1 co-immunoprecipitates APOPTOSIS AND SPLICING ASSOCIATED PROTEIN (ASAP) complex and PRC1. Here, we show that ASAP and PRC1 are necessary for co-transcriptional repression and chromatin regulation at FLC. ASAP mutants affect FLC transcription in warm conditions, but the rate of FLC silencing in the cold is unaffected. PRC1-mediated H2Aub accumulation increases at the FLC nucleation region during cold, but unlike the PRC2-delivered H3K27me3, does not spread across the locus. H2Aub thus involved in the transition to epigenetic silencing at FLC, facilitating H3K27me3 accumulation and long-term epigenetic memory. Overall, our work highlights the importance of VAL1 as an assembly platform co-ordinating activities necessary for epigenetic silencing at FLC. Cold-induced silencing of Arabidopsis FLC requires the binding of VAL1 to an intronic motif. Here, the authors show that ASAP and PRC1, two interacting partner complexes of VAL1, mediate co-transcriptional repression and chromatin modulation to effectively co-ordinate different steps in FLC silencing.
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影响因子：
9.2
作者：
Deka B;Singh KK
通讯作者：
Singh KK
影响因子：
9.3
作者：
Berry S;Dean C;Howard M
通讯作者：
Howard M
DOI：
10.1093/plcell/koab139
发表时间：
2021-08-31
期刊：
The Plant cell
影响因子：
--
作者：
Baile F;Merini W;Hidalgo I;Calonje M
通讯作者：
Calonje M
DOI：
10.1073/pnas.1419030111
发表时间：
2014-11-11
影响因子：
11.1
作者：
Csorba, Tibor;Questa, Julia I.;Dean, Caroline
通讯作者：
Dean, Caroline
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
Dean, C