Probing mechanoregulation of neuronal differentiation by plasma lithography patterned elastomeric substrates.
Probing mechanoregulation of neuronal differentiation by plasma lithography patterned elastomeric substrates.
复制标题
通过等离子光刻的弹性弹性底物对神经元分化的探测机械调节。
DOI：
10.1038/srep06965
复制
发表时间：
2014-11-07
影响因子：
4.6
通讯作者：
Wong PK
中科院分区：
文献类型：
--
作者：
Nam KH;Jamilpour N;Mfoumou E;Wang FY;Zhang DD;Wong PK
细胞感知和解释微环境中的机械信号，包括细胞与细胞和细胞与底物的相互作用，共同调节各种生理功能。了解这些机械因素对细胞行为的影响对于基础细胞生物学和再生医学新策略的发展至关重要。在这里，我们演示了弹性体衬底上的等离子体光刻图形，以阐明机械提示对神经元分化和神经发生的影响。神经母细胞瘤细胞在等离子体处理的区域形成神经球，几何上将细胞限制在两周以上。交联剂浓度同时控制弹性体弹性体的弹性模量。通过控制不同细胞接种密度的神经元球体的大小，研究了细胞与基质的机械相互作用。这些物理线索被证明与局灶性粘连的形成、轴突生长和神经母细胞瘤的形态有关。通过对这些线索的系统调整，以及计算生物力学分析，我们展示了底物弹性和细胞大小之间相互关联的机械调节效应。综上所述，我们的结果表明，神经母细胞瘤细胞的神经元分化和神经发生是通过细胞-底物的机械相互作用来共同调节的。
Cells sense and interpret mechanical cues, including cell-cell and cell-substrate interactions, in the microenvironment to collectively regulate various physiological functions. Understanding the influences of these mechanical factors on cell behavior is critical for fundamental cell biology and for the development of novel strategies in regenerative medicine. Here, we demonstrate plasma lithography patterning on elastomeric substrates for elucidating the influences of mechanical cues on neuronal differentiation and neuritogenesis. The neuroblastoma cells form neuronal spheres on plasma-treated regions, which geometrically confine the cells over two weeks. The elastic modulus of the elastomer is controlled simultaneously by the crosslinker concentration. The cell-substrate mechanical interactions are also investigated by controlling the size of neuronal spheres with different cell seeding densities. These physical cues are shown to modulate with the formation of focal adhesions, neurite outgrowth, and the morphology of neuroblastoma. By systematic adjustment of these cues, along with computational biomechanical analysis, we demonstrate the interrelated mechanoregulatory effects of substrate elasticity and cell size. Taken together, our results reveal that the neuronal differentiation and neuritogenesis of neuroblastoma cells are collectively regulated via the cell-substrate mechanical interactions.
登录
查看更多内容
影响因子：
4.1
作者：
Kullenberg, Johanna;Rosatini, Federica;Domenici, Claudio
通讯作者：
Domenici, Claudio
影响因子：
4.3
作者：
Long J;Junkin M;Wong PK;Hoying J;Deymier P
通讯作者：
Deymier P
影响因子：
3.5
作者：
Das, Eashita;Bhattacharyya, Nitai Pada
通讯作者：
Bhattacharyya, Nitai Pada
影响因子：
3.7
作者：
Gutierrez E;Tkachenko E;Besser A;Sundd P;Ley K;Danuser G;Ginsberg MH;Groisman A
通讯作者：
Groisman A
影响因子：
4
作者：
Junkin, Michael;Leung, Siu Ling;Wong, Pak Kin
通讯作者：
Wong, Pak Kin