Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis.
Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis.
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DOI：
10.1186/1471-2164-15-943
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发表时间：
2014-10-29
期刊：
影响因子：
4.4
通讯作者：
Felipe MS
中科院分区：
文献类型：
--
作者：
Teixeira MM;de Almeida LG;Kubitschek-Barreira P;Alves FL;Kioshima ES;Abadio AK;Fernandes L;Derengowski LS;Ferreira KS;Souza RC;Ruiz JC;de Andrade NC;Paes HC;Nicola AM;Albuquerque P;Gerber AL;Martins VP;Peconick LD;Neto AV;Chaucanez CB;Silva PA;Cunha OL;de Oliveira FF;dos Santos TC;Barros AL;Soares MA;de Oliveira LM;Marini MM;Villalobos-Duno H;Cunha MM;de Hoog S;da Silveira JF;Henrissat B;Niño-Vega GA;Cisalpino PS;Mora-Montes HM;Almeida SR;Stajich JE;Lopes-Bezerra LM;Vasconcelos AT;Felipe MS
孢子菌属真菌包括至少四种人类致病种。其中一种巴西孢子虫是巴西正在发生的重大人畜共患孢子虫病暴发的致病因子。在其他地方，sapronose是由S. schenckii和S. globosa引起的。本比较基因组学研究的主要目的是：1)探索申克孢子虫和巴西孢子虫毒力因子的存在；2)比较猫传播、人猫均可感染的巴西S. brasiliensis与以人为主要病原体的申克S. schenckii；3)将这两个物种与其他具有类似热二态行为的人类病原体（Onygenales）和其他植物相关的sordariomytes进行比较。对申氏链球菌和巴西孢子虫基因组分别进行了17x和20x的焦序测序，覆盖面积分别为32.3 Mb和33.2 Mb。对基因组比对结果显示，两种物种高度同源，平均序列同源性为97.5%。系统基因组学分析表明，这两个物种大约在3.8-4.9 MYA分化，表明这是一个最近的物种形成事件。转座元件分别占申氏孢子和巴西孢子基因组的0.34%和0.62%，gypsylike元件的扩增反映了巴西孢子基因组中重复元件的积累。线粒体基因组比较表明，巴西螺中只存在i族内含子编码归巢内切酶（HE 's）。对孢子菌谱系中蛋白家族扩增和收缩的分析显示，含有LysM结构域的蛋白、小gtpase、PKS 1型和富含白细胞的蛋白扩增。相比之下，与其他索达菌和二态真菌病原体相比，观察到缺乏与植物腐烂相关的多糖裂解酶基因，这表明从植物致病性或腐坏性的进化适应到动物致病性的生活方式。比较基因组数据表明，孢子丝菌谱系从植物关联到哺乳动物寄生的独特生态转变，这有助于理解环境相互作用如何影响真菌的毒力。此外，与其他二态真菌相比发现的显著差异表明，这些与植物相关的sordariomyetes近亲的二态性是趋同进化的一种情况，强调了这种形态发生变化在真菌发病机制中的重要性。本文的在线版本（doi:10.1186/1471-2164-15-943）包含补充材料，可供授权用户使用。
The fungal genus Sporothrix includes at least four human pathogenic species. One of these species, S. brasiliensis, is the causal agent of a major ongoing zoonotic outbreak of sporotrichosis in Brazil. Elsewhere, sapronoses are caused by S. schenckii and S. globosa. The major aims on this comparative genomic study are: 1) to explore the presence of virulence factors in S. schenckii and S. brasiliensis; 2) to compare S. brasiliensis, which is cat-transmitted and infects both humans and cats with S. schenckii, mainly a human pathogen; 3) to compare these two species to other human pathogens (Onygenales) with similar thermo-dimorphic behavior and to other plant-associated Sordariomycetes. The genomes of S. schenckii and S. brasiliensis were pyrosequenced to 17x and 20x coverage comprising a total of 32.3 Mb and 33.2 Mb, respectively. Pair-wise genome alignments revealed that the two species are highly syntenic showing 97.5% average sequence identity. Phylogenomic analysis reveals that both species diverged about 3.8-4.9 MYA suggesting a recent event of speciation. Transposable elements comprise respectively 0.34% and 0.62% of the S. schenckii and S. brasiliensis genomes and expansions of Gypsy-like elements was observed reflecting the accumulation of repetitive elements in the S. brasiliensis genome. Mitochondrial genomic comparisons showed the presence of group-I intron encoding homing endonucleases (HE’s) exclusively in S. brasiliensis. Analysis of protein family expansions and contractions in the Sporothrix lineage revealed expansion of LysM domain-containing proteins, small GTPases, PKS type1 and leucin-rich proteins. In contrast, a lack of polysaccharide lyase genes that are associated with decay of plants was observed when compared to other Sordariomycetes and dimorphic fungal pathogens, suggesting evolutionary adaptations from a plant pathogenic or saprobic to an animal pathogenic life style. Comparative genomic data suggest a unique ecological shift in the Sporothrix lineage from plant-association to mammalian parasitism, which contributes to the understanding of how environmental interactions may shape fungal virulence. . Moreover, the striking differences found in comparison with other dimorphic fungi revealed that dimorphism in these close relatives of plant-associated Sordariomycetes is a case of convergent evolution, stressing the importance of this morphogenetic change in fungal pathogenesis. The online version of this article (doi:10.1186/1471-2164-15-943) contains supplementary material, which is available to authorized users.
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影响因子：
6.7
作者：
Bueter CL;Specht CA;Levitz SM
通讯作者：
Levitz SM
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
DOI：
10.1007/s004380051046
发表时间：
1999-07-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Alexander, NJ;McCormick, SP;Hohn, TM
通讯作者：
Hohn, TM
影响因子：
5.8
作者：
Almeida-Paes R;Frases S;Fialho Monteiro PC;Gutierrez-Galhardo MC;Zancopé-Oliveira RM;Nosanchuk JD
通讯作者：
Nosanchuk JD
影响因子：
2.5
作者：
CUMMINGS, DJ;MCNALLY, KL;MATSUURA, ET
通讯作者：
MATSUURA, ET