The influence of skeletal muscle anisotropy on electroporation: in vivo study and numerical modeling.
The influence of skeletal muscle anisotropy on electroporation: in vivo study and numerical modeling.
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DOI：
10.1007/s11517-010-0614-1
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发表时间：
2010-07
影响因子：
3.2
通讯作者：
Miklavcic, Damijan
中科院分区：
文献类型：
--
作者：
Corovic, Selma;Zupanic, Anze;Kranjc, Simona;Al Sakere, Bassim;Leroy-Willig, Anne;Mir, Lluis M.;Miklavcic, Damijan
关键词：
本研究的目的是从理论和实验上研究小鼠胫骨颅肌的电穿孔，并确定所需的可逆电穿孔阈值的平行和垂直方向的施加电场相对于肌纤维。我们的研究是基于我们建立的三维逼真的数值模型计算的局部电场，以及电穿孔肌肉组织的体内可视化。我们确定了组织中肌细胞的电穿孔取决于所施加电场的方向;确定了平行和垂直方向的局部电场阈值（脉冲参数：8 × 100 μs，1 Hz）分别为80 V/cm和200 V/cm。我们的研究结果可能是有用的电场参数在骨骼肌电穿孔的控制，可用于治疗计划的电穿孔为基础的治疗，如基因治疗，基因疫苗接种，电化学治疗。
The aim of this study was to theoretically and experimentally investigate electroporation of mouse tibialis cranialis and to determine the reversible electroporation threshold values needed for parallel and perpendicular orientation of the applied electric field with respect to the muscle fibers. Our study was based on local electric field calculated with three-dimensional realistic numerical models, that we built, and in vivo visualization of electroporated muscle tissue. We established that electroporation of muscle cells in tissue depends on the orientation of the applied electric field; the local electric field threshold values were determined (pulse parameters: 8 × 100 μs, 1 Hz) to be 80 V/cm and 200 V/cm for parallel and perpendicular orientation, respectively. Our results could be useful electric field parameters in the control of skeletal muscle electroporation, which can be used in treatment planning of electroporation based therapies such as gene therapy, genetic vaccination, and electrochemotherapy.
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影响因子：
4.2
作者：
Andre, F. M.;Gehl, J.;Mir, L. M.
通讯作者：
Mir, L. M.
影响因子：
3.4
作者：
Miklavcic, D;Beravs, K;Sersa, G
通讯作者：
Sersa, G
影响因子：
4.6
作者：
Pavselj, N;Bregar, Z;Miklavcic, D
通讯作者：
Miklavcic, D
影响因子：
5
作者：
Mir, LM
通讯作者：
Mir, LM
DOI：
10.1073/pnas.96.8.4262
发表时间：
1999-04-13
影响因子：
11.1
作者：
Mir, LM;Bureau, MF;Scherman, D
通讯作者：
Scherman, D