Contribution of Interneuron Subtype-Specific GABAergic Signaling to Emergent Sensory Processing in Mouse Somatosensory Whisker Barrel Cortex.
Contribution of Interneuron Subtype-Specific GABAergic Signaling to Emergent Sensory Processing in Mouse Somatosensory Whisker Barrel Cortex.
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DOI：
10.1093/cercor/bhab363
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发表时间：
2022-06-07
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--
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哺乳动物的新皮层对于感觉信息的有意识处理是重要的，平衡的谷氨酸能和氨基丁酸能信号是这一功能的基础。然而，尽管对早期gaba能中间神经元（IN）回路的了解越来越深入，但人们对这种相互作用是如何产生的知之甚少。为了研究这一点，我们评估了特定的INs对小鼠须桶皮层感觉加工发展的贡献，特别是INs在早期速度编码和感觉适应中的作用。在野生型动物中，速度加工和适应性早在可塑性的第4层关键时期就出现了，并在这一时期表现出细化，从而导致活跃的拂动开始。为了测试IN亚型的作用，我们有条件地沉默了生长抑素（SST）或血管活性肠肽（VIP） INs中动作电位依赖性GABA的释放。这些基因操作以年龄和层依赖的方式影响自发和感觉诱发的皮层活动。在对照组幼崽中观察到，沉默SST + INs减少了早期自发活动，并消除了感觉适应的促进作用。相比之下，VIP + In沉默对主动搅拌的开始有影响。沉默任何一种IN亚型对速度编码没有影响。我们的研究结果表明，这些IN亚型在出生后的最初几周内有助于早期感觉加工。
Mammalian neocortex is important for conscious processing of sensory information with balanced glutamatergic and GABAergic signaling fundamental to this function. Yet little is known about how this interaction arises despite increasing insight into early GABAergic interneuron (IN) circuits. To study this, we assessed the contribution of specific INs to the development of sensory processing in the mouse whisker barrel cortex, specifically the role of INs in early speed coding and sensory adaptation. In wild-type animals, both speed processing and adaptation were present as early as the layer 4 critical period of plasticity and showed refinement over the period leading to active whisking onset. To test the contribution of IN subtypes, we conditionally silenced action-potential-dependent GABA release in either somatostatin (SST) or vasoactive intestinal peptide (VIP) INs. These genetic manipulations influenced both spontaneous and sensory-evoked cortical activity in an age- and layer-dependent manner. Silencing SST + INs reduced early spontaneous activity and abolished facilitation in sensory adaptation observed in control pups. In contrast, VIP + IN silencing had an effect towards the onset of active whisking. Silencing either IN subtype had no effect on speed coding. Our results show that these IN subtypes contribute to early sensory processing over the first few postnatal weeks.
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Bale MR;Ince RA;Santagata G;Petersen RS
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