Probing RNA translocases with DNA.

Probing RNA translocases with DNA.
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用 DNA 探测 RNA 易位酶。

DOI:
10.1007/978-1-4939-2214-7_17
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发表时间:
2015
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Raney,KevinD
Raney,KevinD
中科院分区:
--
文献类型:
--
作者:
Reynolds,KimberlyA;Raney,VeronicaM;Raney,KevinD

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For some helicases, it is possible to investigate RNA translocase activity on DNA substrates because the enzyme acts on both substrates. Potassium permanganate (KMnO4) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins. Combining footprinting methods with rapid mixing methods that utilize a chemical quench-flow instrument can enable tracking of the translocase with nucleotide resolution.
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