NADP-Dependent Malic Enzyme 1 Participates in the Abscisic Acid Response in Arabidopsis thaliana.
NADP-Dependent Malic Enzyme 1 Participates in the Abscisic Acid Response in Arabidopsis thaliana.
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DOI：
10.3389/fpls.2018.01637
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发表时间：
2018
影响因子：
5.6
通讯作者：
Saigo M
中科院分区：
文献类型：
--
作者：
Arias CL;Pavlovic T;Torcolese G;Badia MB;Gismondi M;Maurino VG;Andreo CS;Drincovich MF;Gerrard Wheeler MC;Saigo M
拟南芥具有三个胞质（NADP-ME 1 -3）和一个质体（NADP-ME 4）NADP依赖的苹果酸酶。NADP-ME 2和-ME 4显示组成型表达，与NADP-ME 1和-ME 3相反，NADP-ME 1和-ME 3仅限于特定组织。在这里，我们表明，NADP-ME 1的转录本和蛋白质在正常的营养生长过程中几乎检测不到，但逐渐增加，并达到高于其他亚型在种子发育的最后阶段的水平。因此，在敲除nadp-me 1成熟种子中，总NADP-ME活性显著低于野生型成熟种子。nadp-me 1植株的表型分析表明种子生活力和萌发的改变。此外，脱落酸（阿坝）、NaCl和甘露醇处理也能特异性地诱导NADP-ME 1在幼苗中的积累。与此一致，NADP-ME 1植物表现出较弱的反应，主根和侧根长度和气孔开放阿坝的存在。这些结果表明NADP-ME 1在种子发育过程中以及对水分亏缺胁迫的响应中起着专门的作用，与阿坝信号有关。
Arabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic (NADP-ME4) NADP-dependent malic enzymes. NADP-ME2 and -ME4 show constitutive expression, in contrast to NADP-ME1 and -ME3, which are restricted to particular tissues. Here, we show that NADP-ME1 transcript and protein were almost undetectable during normal vegetative growth, but gradually increased and reached levels higher than those of the other isoforms in the latest stages of seed development. Accordingly, in knockout nadp-me1 mature seeds the total NADP-ME activity was significantly lower than in wild type mature seeds. The phenotypic analysis of nadp-me1 plants indicated alterations of seed viability and germination. Besides, the treatment with abscisic acid (ABA), NaCl and mannitol specifically induced the accumulation of NADP-ME1 in seedlings. In line with this, nadp-me1 plants show a weaker response of primary and lateral root length and stomatal opening to the presence of ABA. The results suggest that NADP-ME1 plays a specialized role, linked to ABA signaling during the seed development as well as in the response to water deficit stress.
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