Silk film topography directs collective epithelial cell migration.
Silk film topography directs collective epithelial cell migration.
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DOI：
10.1371/journal.pone.0050190
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rosenblatt MI
中科院分区：
文献类型：
--
作者：
Lawrence BD;Pan Z;Rosenblatt MI
The following study provides new insight into how surface topography dictates directed collective epithelial cell sheet growth through the guidance of individual cell movement. Collective cell behavior of migrating human corneal limbal-epithelial cell sheets were studied on highly biocompatible flat and micro-patterned silk film surfaces. The silk film edge topography guided the migratory direction of individual cells making up the collective epithelial sheet, which resulted in a 75% increase in total culture elongation. This was due to a 3-fold decrease in cell sheet migration rate efficiency for movement perpendicular to the topography edge. Individual cell migration direction is preferred in the parallel approach to the edge topography where localization of cytoskeletal proteins to the topography’s edge region is reduced, which results in the directed growth of the collective epithelial sheet. Findings indicate customized biomaterial surfaces may be created to direct both the migration rate and direction of tissue epithelialization.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
Mrksich, Milan
DOI：
10.1073/pnas.93.8.3509
发表时间：
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影响因子：
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通讯作者：
Bissell, MJ