In vivo intracellular recordings of medial septal and diagonal band of Broca neurons: relationships with theta rhythm
In vivo intracellular recordings of medial septal and diagonal band of Broca neurons: relationships with theta rhythm
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布罗卡神经元内侧间隔和对角带的体内细胞内记录：与 θ 节律的关系
DOI：
10.1007/bf00241962
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发表时间：
2004
影响因子：
2
通讯作者：
E. Garcı́a
中科院分区：
文献类型：
--
作者：
C. Barrenechea;M. Pedemonte;A. Nuñez;E. Garcı́a
记录大鼠中隔、布洛卡神经元对角带（MS-DBB）跨膜电位和海马区活动。研究了MS-DBB细胞在大振幅不规则活动和海马θ节律期间，由感觉刺激（如抚摸动物背部的皮毛）或电刺激口桥网状核（RPO）引起。根据它们的放电模式和“细胞内θ”节律的特点，对三种类型的细胞进行了描述。A型神经元在约17 mV的膜电位（Vm）下表现出连续的节律振荡。这些振荡产生有节奏的高频尖峰序列，与海马θ节律相锁。A型细胞在没有海马θ节律的情况下也表现出细胞内的θ节律，提示A型细胞的活性在海马θ发生中起着最重要的作用。B型细胞具有明显的尖峰后超极化和细胞内θ振荡幅度小于A型细胞。C型细胞仅在海马θ节律存在的情况下才表现出峰后去极化和较低振幅的细胞内θ节律。C型神经元可以在去极化（46%的细胞）或超极化（15%的细胞）vm上发射缓慢的峰值。B型和C型细胞胞内用路西法黄染色。尽管B型和C型神经元表现出不同的电生理特性，但它们具有相似的形态形状。RPO电刺激在A型和B型细胞中产生海马θ节律和细胞内θ节律，而在C型细胞中没有，并增加了C型神经元的尖峰率。电刺激穹窿只在B型和C型神经元中引起突触反应，在12%的C型细胞中引起反形反应。这些结果表明，可能大多数A型节律细胞没有接受海马的直接反馈，至少有一些C型细胞是投射神经元。本研究结果表明，MS-DBB神经元Vm的θ节律振荡引起不同的节律放电模式。
Transmembrane potentials from medial septal and diagonal band of Broca (MS-DBB) neurons and hippocampal field activity were recorded in curarized and urethanized rats. MS-DBB cells were studied during large amplitude irregular activity and during hippocampal θ rhythm, elicited by either sensory (i.e. stroking the fur on the animal's back) or electrical stimulation of the reticularis pontis oralis nucleus (RPO). Three types of cells were described according to their firing pattern and the characteristics of their “intracellular θ” rhythm. Type A neurons displayed continuous rhythmic oscillations in the membrane potential (Vm) of approximately 17 mV. These oscillations generated rhythmic high-frequency spike trains which were phase-locked with hippocampal θ rhythm. Type A cells revealed intracellular θ rhythm even in the absence of hippocampal θ rhythm, suggesting that the activity of this type of cell was the most important in hippocampal θ genesis. Type B cells were characterized by marked postspike afterhyperpolarization and intracellular θ oscillations of smaller amplitude than in type A cells. Type C cells revealed a post-spike afterdepolarization and a lower amplitude, intracellular θ rhythm only in the presence of hippocampal θ rhythm. Type C neurons could fire slow spikes at depolarizing (46% of cells) or hyperpolarizing (15% of cells) Vms. Type B and C cells were intracellularly stained with Lucifer yellow. Although type B and C neurons revealed dissimilar electrophysiological properties, they had comparable morphological shapes. RPO electrical stimulation generated hippocampal θ rhythm and intracellular θ rhythm in types A and B cells but not in type C cells, and increased the spike rate in type C neurons. Electrical stimulation of the fornix only evoked synaptic responses in type B and C neurons, with antidromic responses being elicited in 12% of type C cells. These results indicate that probably most of the type A rhythmic cells did not receive direct hippocampal feedback and that at least some type C cells were projecting neurons. The present findings demonstrate that θ rhythm oscillations in the Vm of MS-DBB neurons elicit different rhythmic discharge patterns.
影响因子：
2.5
作者：
MCCORMICK, DA;CONNORS, BW;PRINCE, DA
通讯作者：
PRINCE, DA
对参与海马同步和去同步的上升脑干系统的分析。
影响因子：
2.5
作者：
Vertes,RP
通讯作者：
Vertes,RP
影响因子：
2.5
作者：
GRIFFITH, WH
通讯作者：
GRIFFITH, WH