Effects of colistin and tigecycline on multidrug-resistant Acinetobacter baumannii biofilms: advantages and disadvantages of their combination.
Effects of colistin and tigecycline on multidrug-resistant Acinetobacter baumannii biofilms: advantages and disadvantages of their combination.
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DOI：
10.1038/s41598-021-90732-3
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发表时间：
2021-06-03
影响因子：
4.6
通讯作者：
Ono Y
中科院分区：
文献类型：
--
作者：
Sato Y;Ubagai T;Tansho-Nagakawa S;Yoshino Y;Ono Y
我们研究了粘菌素（CST）和替加环素（TGC）单独或联合使用对生物膜分散和生物膜包埋的多重耐药鲍曼不动杆菌（MDRAB）菌株R1和R2的抗菌作用。CST和TGC均能抑制MDRAB生物膜中细菌的生长。然而，CST和低浓度TGC的组合减弱了抑制作用。在生物膜分散的MDRAB上观察到CST而不是TGC的杀菌效果。值得注意的是，杀菌效果随着CST和高浓度TGC的组合而增加，而它们随着CST和低浓度TGC的组合而减弱。虽然生物膜形成MDRAB随着CST或TGC浓度的增加而减少，但生物膜没有完全破坏。此外，1-2 μg/mL CST和2-4 μg/mL TGC的组合分别增加了菌株R1和R2的生物膜。生物膜包埋的MDRAB被CST根除，但不是TGC。值得注意的是，CST和高浓度TGC的组合的根除效果增加，而CST和低浓度TGC的组合发生衰减。这些结果提供了关于CST和TGC在治疗生物膜相关MDRAB感染中的联合作用的信息。
We investigated the antimicrobial effects of colistin (CST) and tigecycline (TGC), either alone or in combination, on biofilm-dispersed and biofilm-embedded multidrug-resistant Acinetobacter baumannii (MDRAB) strains R1 and R2. The bacterial growth of biofilm-dispersed MDRAB was inhibited by CST or TGC. However, the inhibitory effects were attenuated by a combination of CST and low concentrations of TGC. The bactericidal effects of CST, but not TGC, were observed on biofilm-dispersed MDRAB. Notably, the bactericidal effects increased with a combination of CST and high concentrations of TGC, whereas they were attenuated with the combination of CST and low concentrations of TGC. Although biofilm formation by MDRAB decreased with increasing concentrations of CST or TGC, there was no complete disruption of the biofilms. Additionally, the biofilms increased with a combination of 1–2 μg/mL CST and TGC at 2 μg/mL and 2–4 μg/mL for strains R1 and R2, respectively. Biofilm-embedded MDRAB was eradicated with CST, but not TGC. Notably, the eradication effects increased with a combination of CST and high concentrations of TGC, whereas attenuation happened with the combination of CST and low concentrations of TGC. These results provide information on the combined effects of CST and TGC in the treatment of biofilm-associated MDRAB infection.
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影响因子：
5.7
作者：
Principe, Luigi;D'Arezzo, Silvia;Visca, Paolo
通讯作者：
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影响因子：
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