Cellular Complexity of Hemochorial Placenta: Stem Cell Populations, Insights from scRNA-seq, and SARS-CoV-2 Susceptibility.
Cellular Complexity of Hemochorial Placenta: Stem Cell Populations, Insights from scRNA-seq, and SARS-CoV-2 Susceptibility.
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DOI：
10.1007/s40778-021-00194-6
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发表时间：
2021
影响因子：
1.4
通讯作者：
Wallingford MC
中科院分区：
文献类型：
--
作者：
Mallery CS Jr;Carrillo M;Mei A;Correia-Branco A;Kashpur O;Wallingford MC
胎盘是新生形成的短暂器官，在支持胎儿生长和发育中起关键作用。胎盘的氧、营养和废物通过依赖于血管结构和细胞类型特异性表达和膜转运蛋白定位的过程进行转运。了解胎盘如何发育对母胎医学具有重要意义。这篇综述的目的是研究目前有关胎盘祖细胞群体的信息。单细胞RNA测序（scRNA-seq）的最新进展为胎盘中细胞类型特异性基因表达模式的研究提供了前所未有的深度。到目前为止，已经进行了几项小鼠胎盘scRNA-seq研究，这些研究产生并分析了胚胎日（E）7.0和E12.5之间完全发育的胎盘的胎盘祖细胞和细胞的转录组。结合人类胎盘scRNA-seq数据，其中部分数据是通过科学界的协调研究活动产生的，以了解SARS-CoV-2感染的可能性，这些哺乳动物研究为小鼠和人类中发现的血绒膜胎盘的细胞和分子组成提供了基本的见解。单细胞胎盘的研究促进了对组织驻留干细胞和分子的理解，这些干细胞和分子准备支持母胎交流和营养运输。在此，我们通过回顾胎盘解剖和细胞群为这些最新发现提供背景，并讨论最近的scRNA-seq小鼠胎盘发现。需要进一步的研究来评估胎盘干细胞在开发治疗伤口愈合和疾病的新治疗方法中的效用。
The placenta is a transient organ that forms de novo and serves a critical role in supporting fetal growth and development. Placental oxygen, nutrients, and waste are transported through processes that depend on vascular structure and cell type-specific expression and localization of membrane transporters. Understanding how the placenta develops holds great significance for maternal–fetal medicine. The purpose of this review is to examine current information regarding placental progenitor populations. Recent advancements in single-cell RNA sequencing (scRNA-seq) provide unprecedented depth for the investigation of cell type-specific gene expression patterns in the placenta. Thus far, several mouse placenta scRNA-seq studies have been conducted which produced and analyzed transcriptomes of placental progenitors and cells of the fully developed placenta between embryonic day (E) 7.0 and E12.5. Together with human placenta scRNA-seq data which, in part, has been produced through coordinated research campaigns in the scientific community to understand the potential for SARS-CoV-2 infection, these mammalian studies lend fundamental insight into the cellular and molecular composition of hemochorial placentae found in both mouse and human. Single-cell placenta research has advanced understanding of tissue-resident stem cells and molecules that are poised to support maternal–fetal communication and nutrient transport. Herein, we provide context for these recent findings by reviewing placental anatomy and cell populations, and discuss recent scRNA-seq mouse placenta findings. Further research is needed to evaluate the utility of placental stem cells in the development of new therapeutic approaches for the treatment of wound healing and disease.
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DOI：
10.3791/54150
发表时间：
2016-06-17
期刊：
Journal of visualized experiments : JoVE
影响因子：
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作者：
Fang JS;Gritz EC;Marcelo KL;Hirschi KK
通讯作者：
Hirschi KK
DOI：
10.1530/rep-20-0231
发表时间：
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期刊：
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影响因子：
7.1
作者：
Cui, D.;Liu, Y.;Yang, H.
通讯作者：
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影响因子：
64.8
作者：
Dickinson ME;Flenniken AM;Ji X;Teboul L;Wong MD;White JK;Meehan TF;Weninger WJ;Westerberg H;Adissu H;Baker CN;Bower L;Brown JM;Caddle LB;Chiani F;Clary D;Cleak J;Daly MJ;Denegre JM;Doe B;Dolan ME;Edie SM;Fuchs H;Gailus-Durner V;Galli A;Gambadoro A;Gallegos J;Guo S;Horner NR;Hsu CW;Johnson SJ;Kalaga S;Keith LC;Lanoue L;Lawson TN;Lek M;Mark M;Marschall S;Mason J;McElwee ML;Newbigging S;Nutter LM;Peterson KA;Ramirez-Solis R;Rowland DJ;Ryder E;Samocha KE;Seavitt JR;Selloum M;Szoke-Kovacs Z;Tamura M;Trainor AG;Tudose I;Wakana S;Warren J;Wendling O;West DB;Wong L;Yoshiki A;International Mouse Phenotyping Consortium;Jackson Laboratory;Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris (ICS);Charles River Laboratories;MRC Harwell;Toronto Centre for Phenogenomics;Wellcome Trust Sanger Institute;RIKEN BioResource Center;MacArthur DG;Tocchini-Valentini GP;Gao X;Flicek P;Bradley A;Skarnes WC;Justice MJ;Parkinson HE;Moore M;Wells S;Braun RE;Svenson KL;de Angelis MH;Herault Y;Mohun T;Mallon AM;Henkelman RM;Brown SD;Adams DJ;Lloyd KC;McKerlie C;Beaudet AL;Bućan M;Murray SA
通讯作者：
Murray SA
ACE2和TMPRSS2的单细胞表达曲线揭示了SARS-COV-2的潜在垂直传播和胎儿感染。
DOI：
10.18632/aging.104015
发表时间：
2020-10-26
期刊：
Aging
影响因子：
--
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Leng Q