Overexpression of a WRKY transcription factor McWRKY57-like from Mentha canadensis L. enhances drought tolerance in transgenic Arabidopsis.
Overexpression of a WRKY transcription factor McWRKY57-like from Mentha canadensis L. enhances drought tolerance in transgenic Arabidopsis.
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加拿大薄荷中WRKY类转录因子McWRKY57的过表达提高了转基因拟南芥的抗旱性。
DOI：
10.1186/s12870-023-04213-y
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发表时间：
2023-04-25
影响因子：
5.3
通讯作者：
Liang, Chengyuan
中科院分区：
文献类型：
--
作者：
Bai, Yang;Zhang, Ting;Zheng, Xiaowei;Li, Bingxuan;Qi, Xiwu;Xu, Yu;Li, Li;Liang, Chengyuan
干旱已成为影响农作物生产的主要环境问题。WRKY家族的成员在植物发育和胁迫反应中起着重要作用。然而，它们在薄荷中的作用几乎没有被探索过。在本研究中，我们从薄荷中分离了一个干旱诱导基因McWRKY 57-like，并对其功能进行了研究。该基因编码IIc组WRKY转录因子McWRKY 57-like，其是具有高度保守的WRKY结构域和C2 H2锌指结构的核蛋白，并具有转录因子活性。在薄荷不同组织中以及在甘露醇、NaCl、脱落酸和茉莉酸甲酯处理下检测其表达水平。我们发现，在拟南芥中McWRKY 57样过表达显着增加耐旱性。进一步的研究表明，在干旱胁迫下，McWRKY 57-like过表达植株的叶绿素、可溶性糖、可溶性蛋白和脯氨酸含量均高于野生型植株，而失水率和丙二醛含量低于野生型植株。此外，抗氧化酶过氧化氢酶，超氧化物歧化酶和过氧化物酶的活性增强McWRKY 57类转基因植物。此外，qRT-PCR分析显示，在模拟干旱条件下，与野生型拟南芥相比，干旱相关基因AtRD 29 A、AtRD 29 B、AtRD 20、AtRAB 18、AtCOR 15 A、AtCOR 15 B、AtKIN 2和AtDREB 1A在McWRKY 57-like转基因拟南芥中表达上调。这些数据表明McWRKY 57-like通过调节植物生长、渗透压物质积累和抗氧化酶活性以及胁迫相关基因的表达来赋予转基因拟南芥耐旱性。该研究表明McWRKY 57-like在植物的干旱响应中起着积极的作用。在线版本包含补充材料，可通过10.1186/s12870-023-04213-y获得。
Drought has become a major environmental problem affecting crop production. Members of the WRKY family play important roles in plant development and stress responses. However, their roles in mint have been barely explored. In this study, we isolated a drought-inducible gene McWRKY57-like from mint and investigated its function. The gene encodes a group IIc WRKY transcription factor, McWRKY57-like, which is a nuclear protein with a highly conserved WRKY domain and a C2H2 zinc-finger structure, and has transcription factor activity. Its expression levels were examined in different tissues of mint and under the treatment of mannitol, NaCl, abscisic acid, and methyl jasmonate. We found that McWRKY57-like overexpression in Arabidopsis significantly increased drought tolerance. Further studies showed that under drought stress, McWRKY57-like-overexpressing plants had higher chlorophyll, soluble sugar, soluble protein, and proline contents but lower water loss rate and malondialdehyde content than wild-type plants. Moreover, the activities of antioxidant enzymes catalase, superoxide dismutase, and peroxidase were enhanced in McWRKY57-like transgenic plants. Furthermore, qRT-PCR analysis revealed that the drought-related genes AtRD29A, AtRD29B, AtRD20, AtRAB18, AtCOR15A, AtCOR15B, AtKIN2, and AtDREB1A were upregulated in McWRKY57-like transgenic plants than in wild-type Arabidopsis under simulated drought conditions. These data demonstrated that McWRKY57-like conferred drought tolerance in transgenic Arabidopsis by regulating plant growth, osmolyte accumulation and antioxidant enzyme activities, and the expression of stress-related genes. The study indicates that McWRKY57-like plays a positive role in drought response in plants. The online version contains supplementary material available at 10.1186/s12870-023-04213-y.
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