Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.
Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.
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DOI：
10.3390/ijms19041172
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发表时间：
2018-04-12
影响因子：
5.6
通讯作者：
Kwok JCF
中科院分区：
文献类型：
--
作者：
Irvine SF;Kwok JCF
神经元周网（Perineuronalnets，PNNs）是中枢神经系统中神经元亚群周围的细胞外基质结构，调节可塑性。酶促去除PNN成功地增强了可塑性，从而增强了功能恢复，特别是在脊髓损伤模型中。虽然各种脑区域内的PNN已经得到了很好的研究，但脊髓中的大部分组成和相关群体仍是未知的。我们的目的是调查的PNN神经元参与这种功能性运动恢复的人口。用胆碱乙酰转移酶（标记运动神经元）、花紫藤凝集素（WFA）和硫酸软骨素蛋白聚糖（CSPGs）（包括聚集蛋白聚糖）标记PNNs，进行免疫组化，以确定大鼠脊髓运动神经元（Mns）PNNs的分子异质性。CSPG阳性PNN包围约70-80%的MN。使用WFA，只有~60%的CSPG阳性PNN与WFA共同定位在脊髓Mns中，而~15-30%的Mns显示CSPG阳性但WFA阴性的PNN。选择性标记显示聚集蛋白聚糖包围了约90%的α Mns。结果表明：（1）聚集蛋白聚糖比WFA更好地标记脊髓PNN，（2）脊髓和皮质之间PNN组成及其相关神经元群体存在差异。深入了解PNN及其在脊髓运动池中的分子异质性的作用，可以帮助设计有针对性的策略，以增强损伤后的功能恢复。
Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity. Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in spinal cord injury models. While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown. We aim to investigate the populations of PNN neurones involved in this functional motor recovery. Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns). CSPG-positive PNNs surrounded ~70–80% of Mns. Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15–30% of Mns showed CSPG-positive but WFA-negative PNNs. Selective labelling revealed that aggrecan encircled ~90% of alpha Mns. The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex. Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.
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影响因子：
3.3
作者：
Fader SM;Imaizumi K;Yanagawa Y;Lee CC
通讯作者：
Lee CC
影响因子：
4.2
作者：
BIGNAMI, A;PERIDES, G;RAHEMTULLA, F
通讯作者：
RAHEMTULLA, F
软骨素酶ABC促进脊髓损伤后完整和受伤的脊柱系统的发芽。
DOI：
10.1523/jneurosci.2980-06.2006
发表时间：
2006-10-18
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Barritt AW;Davies M;Marchand F;Hartley R;Grist J;Yip P;McMahon SB;Bradbury EJ
通讯作者：
Bradbury EJ
影响因子：
14.8
作者：
Gama, Cristal I.;Tully, Sarah E.;Hsieh-Wilson, Linda C.
通讯作者：
Hsieh-Wilson, Linda C.
影响因子：
5.3
作者：
Asher, RA;Morgenstern, DA;Fawcett, JW
通讯作者：
Fawcett, JW