High-Fat-Diet-Evoked Disruption of the Rat Dorsomedial Hypothalamic Clock Can Be Prevented by Restricted Nighttime Feeding.
High-Fat-Diet-Evoked Disruption of the Rat Dorsomedial Hypothalamic Clock Can Be Prevented by Restricted Nighttime Feeding.
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DOI：
10.3390/nu14235034
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发表时间：
2022-11-26
期刊：
影响因子：
5.9
通讯作者：
Lewandowski MH
中科院分区：
文献类型：
--
作者：
Sanetra AM;Palus-Chramiec K;Chrobok L;Jeczmien-Lazur JS;Gawron E;Klich JD;Pradel K;Lewandowski MH
肥胖是现代社会日益严重的健康问题；因此，不仅要研究其负面影响，还要研究其发展机制。它与昼夜节律紊乱的联系是无可争议的，但在细胞水平上仍未得到很好的研究。昼夜节律的食物摄入和代谢是由一组被称为食物可携带振荡器的大脑结构控制的，其中背内侧下丘脑（DMH）似乎受到饮食引起的肥胖的严重影响。在这项研究中，我们评估了短期高脂肪饮食（HFD）对雄性大鼠DMH生理的影响，特别关注其昼夜变化。通过免疫荧光和电生理学，我们发现cfo的免疫反应性和电活动节律在食用HFD 4周后就会被打乱，因此在体重过度增加之前。这表明DMH损伤可能是肥胖发展的一个因素。高热量饮食下的DMH细胞活性在非活动的白天增加，这与食物摄入的节律紊乱相吻合。为了探索它们之间的关系，另一组老鼠进行了限时喂养，只在夜间获得食物。这种方法完全消除了HFD对DMH时钟的破坏性影响，证实了其对动物摄食时间表的依赖性。这些数据强调了暂时调节进食模式对下丘脑中心的食物摄入和代谢调节的重要性，并提出了限时进食作为一种可能的预防在HFD下观察到的昼夜节律失调的方法。
Obesity is a growing health problem for modern society; therefore, it has become extremely important to study not only its negative implications but also its developmental mechanism. Its links to disrupted circadian rhythmicity are indisputable but are still not well studied on the cellular level. Circadian food intake and metabolism are controlled by a set of brain structures referred to as the food-entrainable oscillator, among which the dorsomedial hypothalamus (DMH) seems to be especially heavily affected by diet-induced obesity. In this study, we evaluated the effects of a short-term high-fat diet (HFD) on the physiology of the male rat DMH, with special attention to its day/night changes. Using immunofluorescence and electrophysiology we found that both cFos immunoreactivity and electrical activity rhythms become disrupted after as few as 4 weeks of HFD consumption, so before the onset of excessive weight gain. This indicates that the DMH impairment is a possible factor in obesity development. The DMH cellular activity under an HFD became increased during the non-active daytime, which coincides with a disrupted rhythm in food intake. In order to explore the relationship between them, a separate group of rats underwent time-restricted feeding with access to food only during the nighttime. Such an approach completely abolished the disruptive effects of the HFD on the DMH clock, confirming its dependence on the feeding schedule of the animal. The presented data highlight the importance of a temporally regulated feeding pattern on the physiology of the hypothalamic center for food intake and metabolism regulation, and propose time-restricted feeding as a possible prevention of the circadian dysregulation observed under an HFD.
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影响因子：
29
作者：
Hatori M;Vollmers C;Zarrinpar A;DiTacchio L;Bushong EA;Gill S;Leblanc M;Chaix A;Joens M;Fitzpatrick JA;Ellisman MH;Panda S
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作者：
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通讯作者：
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