Severity of Chagasic Cardiomyopathy Is Associated With Response to a Novel Rapid Diagnostic Test for Trypanosoma cruzi TcII/V/VI.
Severity of Chagasic Cardiomyopathy Is Associated With Response to a Novel Rapid Diagnostic Test for Trypanosoma cruzi TcII/V/VI.
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Chagasic心肌病的严重程度与对锥虫TCII/V/VI的新型快速诊断测试的反应有关。
DOI：
10.1093/cid/ciy121
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发表时间：
2018-08-01
期刊：
影响因子：
--
通讯作者：
Chagas Working Group in Bolivia and Peru
中科院分区：
文献类型：
--
作者：
Bhattacharyya T;Messenger LA;Bern C;Mertens P;Gilleman Q;Zeippen N;Bremer Hinckel BC;Murphy N;Gilman RH;Miles MA;Chagas Working Group in Bolivia and Peru
我们描述了一种新的，即时的，快速诊断检测克氏锥虫TcII/V/VI感染。阳性检测反应与查加斯病心肌病的严重程度相关。重复T。cruzi感染可加重疾病并增强对该TSSApep-II/V/VI表位的免疫应答。 克氏锥虫在美洲引起恰加斯病。感染的结果从终身无症状状态到严重疾病不等。T. cruzi谱系（TcI-TcVI）感染史和预后尚不清楚。我们以前描述了基于肽的谱系特异性酶联免疫吸附试验（ELISA）与锥虫小表面抗原（TSSA）。开发了一种新的快速诊断试验（RDT;查加斯血清K-SeT），其结合了对应于TSSA II/V/VI共同表位的肽，并通过与ELISA的比较进行了验证。来自玻利维亚和秘鲁的患者，包括具有不同心脏病理的个体，以及匹配的母亲和新生儿，然后使用Chagas Sero K-SeT进行测试。在玻利维亚心脏病患者、母亲和新生儿的亚组中，查加斯血清K-SeT和ELISA结果雅阁。在成人慢性感染（n = 121）中，A级严重程度比较（没有查加斯心肌病的证据）与B级（心电图提示查加斯心肌病）和C/D级（左心室射血分数降低;中度/重度查加斯心肌病）的对照显示查加斯血清K-SeT反应性随着严重程度的增加而统计学显著增加（趋势χ2，7.39; P = 0.007）。在秘鲁，查加斯血清K-SeT检测到散发的TcII/V/VI感染。我们建立了一种低成本的RDT，可以取代ELISA用于鉴定TSSA II/V/VI免疫球蛋白G。最重要的是，我们发现对RDT的反应与查加斯心肌病的严重程度相关，因此可能具有预后价值。重复挑战T。cruzi感染可能既加剧疾病进展又增强对TSSApep-II/V/VI表位的免疫应答。
We describe a novel, point-of-care, rapid diagnostic test for Trypanosoma cruzi TcII/V/VI infection. Positive test response is associated with severity of Chagas cardiomyopathy. Repeated T. cruzi infection may exacerbate disease and boost immune response to this TSSApep-II/V/VI epitope. Trypanosoma cruzi causes Chagas disease in the Americas. The outcome of infection ranges from lifelong asymptomatic status to severe disease. Relationship between T. cruzi lineage (TcI-TcVI) infection history and prognosis is not understood. We previously described peptide-based lineage-specific enzyme-linked immunosorbent assay (ELISA) with trypomastigote small surface antigen (TSSA). A novel rapid diagnostic test (RDT; Chagas Sero K-SeT) that incorporates a peptide that corresponds to the TSSA II/V/VI common epitope was developed and validated by comparison with ELISA. Patients from Bolivia and Peru, including individuals with varying cardiac pathology, and matched mothers and neonates, were then tested using Chagas Sero K-SeT. Chagas Sero K-SeT and ELISA results, with a Bolivian subset of cardiac patients, mothers, and neonates, were in accord. In adult chronic infections (n = 121), comparison of severity class A (no evidence of Chagas cardiomyopathy) with class B (electrocardiogram suggestive of Chagas cardiomyopathy) and class C/D (decreased left ventricular ejection fraction; moderate/severe Chagas cardiomyopathy) revealed a statistically significant increase in Chagas Sero K-SeT reactivity with increasing severity (χ2 for trend, 7.39; P = .007). In Peru, Chagas Sero K-SeT detected the sporadic TcII/V/VI infections. We developed a low cost RDT that can replace ELISA for identification of TSSA II/V/VI immunoglobulin G. Most importantly, we show that response to this RDT is associated with severity of Chagas cardiomyopathy and thus may have prognostic value. Repeated challenge with T. cruzi infection may both exacerbate disease progression and boost the immune response to the TSSApep-II/V/VI epitope.
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影响因子：
11.8
作者：
Messenger, Louisa A.;Gilman, Robert H.;Bern, Caryn
通讯作者：
Bern, Caryn
影响因子：
3.2
作者：
Guhl, Felipe;David Ramirez, Juan
通讯作者：
David Ramirez, Juan
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DOI：
10.1007/s10096-025-05156-y
发表时间：
2025-05-14
影响因子：
3.000
作者：
Elin Lindberg;Ann Hermansson;David Nygren;Marie Gisselsson-Solén
通讯作者：
Marie Gisselsson-Solén
影响因子：
9.4
作者：
Balouz, Virginia;Melli, Luciano J.;Altcheh, Jaime
通讯作者：
Altcheh, Jaime
影响因子：
9.4
作者：
Sanchez-Camargo, Claudia L.;Albajar-Vinas, Pedro;Flevaud, Laurence
通讯作者：
Flevaud, Laurence