Bmp-4 requires the presence of the digits to initiate programmed cell death in limb interdigital tissues.
Bmp-4 requires the presence of the digits to initiate programmed cell death in limb interdigital tissues.
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Bmp-4 需要手指的存在来启动肢体指间组织中的程序性细胞死亡。
作者：
M. Tang;A. Leung;W. Kwong;P. Chow;J. Chan;V. Ngô;M. Li;K. K. Lee
在小鼠中研究了BMP-4对趾间细胞死亡的影响。将负载有重组BMP-4蛋白的Affi-Gel珠移植到第12.5天后肢的趾间组织中，子宫外。已经确定BMP-4可以诱导早熟的趾间细胞死亡。采用原位杂交技术，建立了BMP-4和ALK-6受体的表达模式。这两个基因被发现共表达在12.5和13的趾间区。5-日后肢。这表明BMP-4可能以自分泌方式起作用。我们还研究了BMP-4对12.5天趾间组织培养的影响。在所有的标本中，趾间组织产生软骨，而不是参与细胞死亡。在趾间培养物中加入外源性BMP-4并不诱导细胞凋亡，而是增强软骨形成。BMP-4在体外和子宫外的作用之间的差异归因于手指的存在。在体外实验中，当一个侧趾附着在趾间组织上时，BMP-4促进了细胞凋亡而不是软骨形成。总之，结果表明，BMP-4是一种多功能蛋白，其对指间组织的作用依赖于手指的调节作用。
The effects of Bmp-4 on interdigital cell death were investigated in the mouse. Affi-Gel beads, loaded with recombinant Bmp-4 protein, were transplanted into the interdigital tissues of day 12.5 hindlimb, ex utero. It was established that Bmp-4 could induce precocious interdigital cell death. Using in situ hybridization, the expression patterns of bmp-4 and alk-6 receptor were established. Both genes were found coexpressed in the interdigital region of 12.5- and 13. 5-day hindlimbs. This suggests that Bmp-4 may act in an autocrine fashion. We have also studied the effects of Bmp-4 on 12.5-day interdigital tissue cultures. In all specimens examined, the interdigital tissues produced cartilage instead of participating in cell death. The addition of exogenous Bmp-4 to the interdigital cultures did not induce apoptosis but instead enhanced chondrogenesis. The discrepancy between the effects of Bmp-4 in vitro and ex utero was attributed to the presence of digits. When a flanking digit was left attached to the interdigital tissues, in vitro, Bmp-4 promoted apoptosis instead of chondrogenesis. In sum, the results suggest that Bmp-4 is a multifunctional protein and its effect on the interdigital tissues is dependent on the modulating influence of the digits.
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顶端外胚层脊去除后肢芽中胚层细胞死亡的空间和时间模式。
影响因子：
2.7
作者：
Rowe,DA;Cairns,JM;Fallon,JF
通讯作者：
Fallon,JF
影响因子：
8
作者：
P. tenDijke;H. Ichijo;P. Franzén;P. Schulz;J. Saras;H. Toyoshima;C. Heldin;K. Miyazono
通讯作者：
P. tenDijke;H. Ichijo;P. Franzén;P. Schulz;J. Saras;H. Toyoshima;C. Heldin;K. Miyazono
DOI：
--
发表时间：
1994-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Dijke;Hidetoshi Yamashitas;-T.;Kuber Sampathfl;Reddill;Miguel Estevez;Donald;-L.;Riddle
通讯作者：
P. Dijke;Hidetoshi Yamashitas;-T.;Kuber Sampathfl;Reddill;Miguel Estevez;Donald;-L.;Riddle
影响因子：
2.7
作者：
Dupé, V;Ghyselinck, NB;Mark, M
通讯作者：
Mark, M
外胚层条件培养基和纤连蛋白对肢芽间充质细胞软骨形成影响的比较。
影响因子：
2.7
作者：
Zanetti,NC;Dress,VM;Solursh,M
通讯作者：
Solursh,M