Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells.
Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells.
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外胚层条件培养基和纤连蛋白对肢芽间充质细胞软骨形成影响的比较。
DOI：
10.1016/0012-1606(90)90307-5
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发表时间：
1990
影响因子：
2.7
通讯作者：
Solursh,M
中科院分区：
文献类型：
--
作者：
Zanetti,NC;Dress,VM;Solursh,M
肢芽外胚层可抑制高密度或胶原凝胶培养的肢芽间充质细胞的软骨形成。这种外胚层抗软骨形成的影响被认为是在活体调节肢体软骨和软结缔组织的空间模式中起作用的。我们开发了一种制备含有抗软骨活性的外胚层条件培养液的方法。利用一种简单的生物测定方法，我们研究了外胚层产物的一些特性及其对肢芽间充质细胞的影响。在胶原凝胶上培养的肢芽间充质细胞上，检测外胚层条件培养液对软骨形成的抑制作用。抗软骨的影响包括促进细胞扩散，并被诱导细胞变圆的药物(如细胞松弛素D)缓解。纤维连接蛋白在肢芽间充质细胞胶原凝胶培养中抑制软骨形成和促进细胞铺展的能力与外胚层条件培养液相似。然而，Western印迹分析表明，外胚层条件培养液的抗软骨形成活性不是由于培养液中的纤维连接蛋白所致。与纤维连接蛋白细胞结合域相关的多肽可阻断纤维连接蛋白的抗软骨形成作用，但不能阻断外胚层条件培养液的作用。另一方面，整合素的抗体和硫酸肝素可以减轻纤维连接蛋白和外胚层条件培养液的抗软骨形成作用。外胚层条件培养液的抗软骨作用可能是由整合素和细胞表面硫酸乙酰肝素蛋白多糖介导的，但不直接依赖于纤维连接蛋白介导的细胞扩散。
Limb bud ectoderm inhibits chondrogenesis by limb bud mesenchymal cells cultured at high density or on collagen gels. This ectodermal antichondragenic influence has been postulated to functionin vivoin regulating the spatial patterning of cartilage and soff connective tissue in the limb. We have developed a method for preparing ectoderm-conditioned medium containing antichondrogenic activity. Using a simple bioassay, we have investigated some characteristics of the ectodermal products and their effects on limb bud mesenchymal cells. Inhibition of chondrogenesis by ectoderm-conditioned medium was tested on limb bud mesenchymal cells cultured on collagen gels. The antichondrogenic influence involves enhanced cell spreading and is alleviated by agents, such as cytochalasin D, that induce cell rounding. Fibronectin resembles ectoderm-conditioned medium in its ability to inhibit chondrogenesis and promote cell spreading in collagen gel cultures of limb bud mesenchymal cells. However, Western blot analysis shows that the antichondrogenic activity of ectoderm-conditioned medium is not due to fibronectin in the medium. Peptides related to the fibronectin cell-binding domain block the antichondrogenic effect of fibronectin, but not that of ectoderm-conditioned medium. On the other hand, an antibody to an integrin, as well as heparan sulfate, alleviates the antichondrogenic effects of both fibronectin and ectoderm-conditioned medium. The antichondrogenic effect of ectoderm-conditioned medium may be mediated by an integrin and by a cell surface heparan sulfate proteoglycan, but it does not depend directly upon fibronectin-mediated cell spreading.
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影响因子：
2.9
作者：
SWALLA, BJ;SOLURSH, M
通讯作者：
SOLURSH, M
细胞外基质介导体外软骨形成的上皮效应。
影响因子：
2.7
作者：
Solursh,M;Jensen,KL;Zanetti,NC;Linsenmayer,TF;Reiter,RS
通讯作者：
Reiter,RS
微团培养中鸡肢芽间充质的生肌潜力。
DOI：
10.1007/bf00211829
发表时间：
1992
期刊：
Anatomy and embryology
影响因子：
--
作者：
Archer,CW;Langille,RM;Teran,MA;Solursh,M
通讯作者：
Solursh,M
DOI：
10.1083/jcb.104.3.573
发表时间：
1987-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Singer II;Kawka DW;Scott S;Mumford RA;Lark MW
通讯作者：
Lark MW
纤连蛋白与糖胺聚糖相互作用的表征以及活性蛋白水解片段的鉴定。
影响因子：
4.8
作者：
K. Yamada;D. Kennedy;K. Kimata;R. Pratt
通讯作者：
R. Pratt