Pre-infestation of Tomato Plants by Aphids Modulates Transmission-Acquisition Relationship among Whiteflies, Tomato Yellow Leaf Curl Virus (TYLCV) and Plants.
Pre-infestation of Tomato Plants by Aphids Modulates Transmission-Acquisition Relationship among Whiteflies, Tomato Yellow Leaf Curl Virus (TYLCV) and Plants.
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DOI：
10.3389/fpls.2017.01597
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发表时间：
2017
影响因子：
5.6
通讯作者：
Ge F
中科院分区：
文献类型：
--
作者：
Tan XL;Chen JL;Benelli G;Desneux N;Yang XQ;Liu TX;Ge F
植物的食草性防御系统主要受茉莉酸盐（JA）和水杨酸盐（SA）信号通路的调控。这种防御机制可能影响昆虫的摄食动态，也可能影响病毒、植物和病媒昆虫之间的传播-获取关系。在番茄-白蝇-番茄黄卷叶病毒（TYLCV）生物学模型的背景下，我们测试了非媒介昆虫（桃蚜）预侵染植物对媒介昆虫（白蝇烟粉虱）取食动力学和病毒传播获取的影响。我们发现，蚜虫的食草期为0-48小时，会导致寄主植物（根、茎和叶）中病毒浓度的短暂全体性增加，在以蚜虫侵染的植物为食的白蝇中也观察到相同的模式。我们利用实时定量PCR技术研究了SA-和ja -信号通路关键基因的表达，并利用电渗透图（EPG）表征了蚜虫预侵染对TYLCV传播（白蝇到番茄）和获取（番茄到白蝇）过程中白蝇取食的影响。蚜前侵染时间（0、24和48 h）对传播期粉虱韧皮部摄食（E2）的影响与采食期粉虱摄食行为（E1、E2和探测时间）的影响相似。此外，我们观察到，在白蝇传播病毒之前，较长的蚜虫预侵染期分别导致SA-和ja -信号通路基因的上调和下调。这些结果表明，蚜虫预侵染对番茄-粉虱- TYLCV系统有显著影响。TYLCV的传播和获取与烟粉虱取食活性呈正相关，均通过SA-和ja -途径介导。传递期TYLCV的浓度分别通过SA-和ja -通路的上调和下调来调节。在习得阶段，这两种途径不一致；SA相关基因表达上调，而JA通路上下游基因表达关系更为复杂。这些发现增强了我们对植物-草食动物-病毒相互作用的理解，这对制定生态无害的害虫和病原体管理计划具有潜在的重要意义。
Herbivory defense systems in plants are largely regulated by jasmonate-(JA) and salicylate-(SA) signaling pathways. Such defense mechanisms may impact insect feeding dynamic, may also affect the transmission-acquisition relationship among virus, plants and vectoring insects. In the context of the tomato – whitefly – Tomato Yellow Leaf Curl Virus (TYLCV) biological model, we tested the impact of pre-infesting plants with a non-vector insect (aphid Myzus persicae) on feeding dynamics of a vector insect (whitefly Bemisia tabaci) as well as virus transmission-acquisition. We showed that an aphid herbivory period of 0–48 h led to a transient systemic increase of virus concentration in the host plant (root, stem, and leaf), with the same pattern observed in whiteflies feeding on aphid-infested plants. We used real-time quantitative PCR to study the expression of key genes of the SA- and JA-signaling pathways, as well as electrical penetration graph (EPG) to characterize the impact of aphid pre-infestation on whitefly feeding during TYLCV transmission (whitefly to tomato) and acquisition (tomato to whitefly). The impact of the duration of aphid pre-infestation (0, 24, or 48 h) on phloem feeding by whitefly (E2) during the transmission phase was similar to that of global whitefly feeding behavior (E1, E2 and probing duration) during the acquisition phase. In addition, we observed that a longer phase of aphid pre-infestation prior to virus transmission by whitefly led to the up-regulation and down-regulation of SA- and JA-signaling pathway genes, respectively. These results demonstrated a significant impact of aphid pre-infestation on the tomato – whitefly – TYLCV system. Transmission and acquisition of TYLCV was positively correlated with feeding activity of B. tabaci, and both were mediated by the SA- and JA-pathways. TYLCV concentration during the transmission phases was modulated by up- and down-regulation of SA- and JA-pathways, respectively. The two pathways were inconsistent during the acquisition phase; SA- related genes were up-regulated, whereas those up- and down-stream of the JA pathway showed a more complex relationship. These findings enhance our understanding of plant – herbivore – virus interactions, which are potentially important for development of ecologically sound pest and pathogen management programs.
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影响因子：
3.4
作者：
Agrawal, AA;Karban, R;Colfer, RG
通讯作者：
Colfer, RG
DOI：
10.1111/j.1365-2338.1995.tb01467.x
发表时间：
1995-01-01
期刊：
Bulletin OEPP
影响因子：
--
作者：
Al-Bitar, L.;Luisoni, E.
通讯作者：
Luisoni, E.
影响因子：
4.8
作者：
Baldin, Edson L. L.;Crotti, Antonio E. M.;Groppo, Milton
通讯作者：
Groppo, Milton
影响因子：
3.2
作者：
Ghanim, M;Morin, S;Czosnek, H
通讯作者：
Czosnek, H
影响因子：
56.9
作者：
Liu, Shu-Sheng;De Barro, P. J.;Wan, Fang-Hao
通讯作者：
Wan, Fang-Hao