Letrozole sensitizes breast cancer cells to ionizing radiation.
Letrozole sensitizes breast cancer cells to ionizing radiation.
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DOI：
10.1186/bcr969
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Pèlegrin A
中科院分区：
文献类型：
--
作者：
Azria D;Larbouret C;Cunat S;Ozsahin M;Gourgou S;Martineau P;Evans DB;Romieu G;Pujol P;Pèlegrin A
放射治疗 (RT) 被认为是乳腺癌手术后的标准治疗选择。来曲唑是一种芳香酶抑制剂，目前正在辅助治疗中进行评估。我们确定了 RT 和来曲唑组合对稳定转染 CYP19 基因的表达芳香酶的乳腺肿瘤细胞系 MCF-7CA 的影响。使用钴 60 源进行照射，剂量范围为 0 至 4 Gy。在存在或不存在来曲唑的情况下将细胞与雄烯二酮一起孵育。使用克隆形成测定、四唑盐比色（MTT）测定和细胞数测定来评估治疗效果。使用流式细胞术进行细胞周期分析。单独放疗时 2 Gy 的存活率为 0.66，放疗加来曲唑时的存活率为 0.44（P = 0.02）。与仅接受放疗的细胞相比，接受放疗（2 和 4 Gy）后 6 天的细胞数测量的 MCF-7CA 细胞生长，在另外用来曲唑（0.7 μM）治疗的细胞中减少了 76%（P = 0.009）。通过细胞数量（P = 0.009）或MTT测定（P = 0.02）评估的生长抑制在联合治疗12天后增加。与单独放疗相比，放疗和来曲唑的组合显着减少了放疗引起的 G2 期停滞，并减少了 S 期细胞的数量，同时细胞重新分布在 G1 期。这些放射生物学结果可能构成同时使用来曲唑和放射作为乳腺癌术后辅助治疗的基础。
Radiotherapy (RT) is considered a standard treatment option after surgery for breast cancer. Letrozole, an aromatase inhibitor, is being evaluated in the adjuvant setting. We determined the effects of the combination of RT and letrozole in the aromatase-expressing breast tumour cell line MCF-7CA, stably transfected with the CYP19 gene. Irradiations were performed using a cobalt-60 source with doses ranging from 0 to 4 Gy. Cells were incubated with androstenedione in the presence or absence of letrozole. Effects of treatment were evaluated using clonogenic assays, tetrazolium salt colorimetric (MTT) assays, and cell number determinations. Cell-cycle analyses were conducted using flow cytometry. The survival fraction at 2 Gy was 0.66 for RT alone and was 0.44 for RT plus letrozole (P = 0.02). Growth of MCF-7CA cells as measured by the cell number 6 days after radiotherapy (2 and 4 Gy) was decreased by 76% in those cells treated additionally with letrozole (0.7 μM) compared with those receiving radiotherapy alone (P = 0.009). Growth inhibition, assessed either by cell number (P = 0.009) or by the MTT assay (P = 0.02), was increased after 12 days of the combination treatment. Compared with radiation alone, the combination of radiation and letrozole produced a significant decrease in radiation-induced G2 phase arrest and a decrease of cells in the S phase, with cell redistribution in the G1 phase. These radiobiological results may form the basis for concurrent use of letrozole and radiation as postsurgical adjuvant therapy for breast cancer.
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影响因子：
168.9
作者：
Overgaard, M;Jensen, MB;Mouridsen, HT
通讯作者：
Mouridsen, HT
影响因子：
105.7
作者：
ONEILL, JS;ELTON, RA;MILLER, WR
通讯作者：
MILLER, WR
影响因子：
158.5
作者：
FISHER, B;BAUER, M;RUSSELL, I
通讯作者：
RUSSELL, I
DOI：
10.1016/0360-3016(78)90185-2
发表时间：
1978-01-01
影响因子：
7
作者：
GOULD, MN;CLIFTON, KH
通讯作者：
CLIFTON, KH
影响因子：
3.8
作者：
SARKARIA, JN;MILLER, EM;MULCAHY, RT
通讯作者：
MULCAHY, RT