Induction of acetylcholine receptor synthesis and aggregation: partial purification of low-molecular-weight activity.
Induction of acetylcholine receptor synthesis and aggregation: partial purification of low-molecular-weight activity.
复制标题
乙酰胆碱受体合成和聚集的诱导：低分子量活性的部分纯化。
DOI：
10.1016/0012-1606(83)90039-8
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发表时间：
1983
影响因子：
2.7
通讯作者：
Fischbach,GD
中科院分区：
文献类型：
--
作者：
Buc-Caron,MH;Nystrom,P;Fischbach,GD
本文研究了鸡脑生理盐水和酸性提取物对培养的鸡肌细胞乙酰胆碱受体总数和受体簇数的影响。培养7天的肌管比培养4天的肌管对脑提取液的反应更快，因此在随后的生物检测中使用了较老的细胞。很大比例的受体诱导活性可溶于2%的三氟乙酸(TFA)，Sephadex G-25层析显示该物质的大小约为1000道尔顿。活性被保留在十八烷基硅基二氧化硅上，并通过TFA-乙腈梯度系统的反相高压液相色谱进一步纯化。在35%至40%的乙腈中洗脱的物质，称为C4018，其效力是生理盐水提取物的500至1000倍。C4018诱导的受体聚集与受体掺入速率的增加有关，可能是与受体合成有关，而不是与受体降解的减少有关。C4018加入培养7天后3h即可检测到掺入量的增加，10h后效果最好。C4018还促进了在加入时已被结合在表膜上的受体的聚集。目前尚不清楚“老”受体的聚集和受体合成增加是否相关，或者这两种现象是否由同一分子介导。
We have studied the effect of saline and acid extracts of chick brain on the total number of acetylcholine (ACh) receptors and the number of receptor clusters in cultured chick muscle cells. Myotubes in 7-day cultures responded more rapidly to brain extract than did myotubes in 4-day cultures, so the older cells were used in subsequent bioassays. A large percentage of the receptor inducing activity was soluble in 2% trifluoroacetic acid (TFA), and this material appeared by Sephadex G-25 chromatography to be about 1000 daltons in size. Activity was retained on octadecasilyl silica and was further purified by reverse-phase high-pressure liquid chromatography using a TFA-acetonitrile gradient system. Material that eluted between 35 and 40% acetonitrile, termed C4018, was 500- to 1000-fold more potent than saline extract. The receptor accumulation induced by C4018was associated with an increased rate of receptor incorporation, presumably receptor synthesis, rather than to a decrease in receptor degradation. An increase in incorporation was detected as early as 3 hr after C4018was added to 7-day cultures and the effect was maximal after 10 hr. C4018also promoted the aggregation of receptors that were already incorporated in the surface membrane at the time to addition. It is not yet known if aggregation of “old” receptors and increased receptor synthesis are related or if the two phenomena are mediated by the same molecule.
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Sciatin：一种肌营养蛋白，可增加培养骨骼肌中乙酰胆碱受体和受体簇的数量。
影响因子：
2.7
作者：
Markelonis,GJ;Oh,TH;Eldefrawi,ME;Guth,L
通讯作者：
Guth,L
培养中突触连接发育过程中神经炎生长锥和靶神经元的形态变化
影响因子：
7.8
作者：
R. Rees;M. Bunge;R. Bunge
通讯作者：
R. Bunge
神经支配对培养的肌肉细胞上乙酰胆碱受体分布的影响。
DOI：
--
发表时间：
1977
期刊：
Journal of Physiology
影响因子：
--
作者：
M. Anderson;M. Cohen;E. Zorychta
通讯作者：
E. Zorychta
肽 — 结构和生物功能：由 E. Gross 和 J. Meienhofer Pierce Chemical Company 编辑；罗克福德，伊利诺伊州，1980 xxxii + 1080 页。
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
A. Darbre
通讯作者：
A. Darbre
影响因子：
2.9
作者：
RAVDIN, P;AXELROD, D
通讯作者：
AXELROD, D