Protein arginine methyltransferase 10 is required for androgen-dependent proliferation of LNCaP prostate cancer cells
Protein arginine methyltransferase 10 is required for androgen-dependent proliferation of LNCaP prostate cancer cells
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LNCaP 前列腺癌细胞雄激素依赖性增殖需要蛋白精氨酸甲基转移酶 10
DOI：
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
H. Inui
中科院分区：
文献类型：
--
作者：
Naoki Harada;T. Takagi;Y. Nakano;R. Yamaji;H. Inui
雄激素受体（AR）信号是前列腺细胞生长的主要调控因子。为了更好地理解AR信号，我们通过酵母双杂交筛选寻找AR相互作用蛋白，并鉴定出蛋白精氨酸甲基转移酶10 （PRMT10）为其中一个相互作用蛋白。PRMT10在生殖组织中高表达，如前列腺。免疫染色显示PRMT10在大鼠前列腺上皮细胞核和间质细胞核中均有表达。在人前列腺癌LNCaP细胞中，PRMT10在存在和不存在双氢睾酮（DHT）的情况下与AR共免疫沉淀。用siRNA敲低PRMT10可降低DHT依赖性LNCaP细胞的生长和前列腺特异性抗原（AR靶基因）的诱导，但AR未明显丧失。DHT可降低PRMT10的mRNA和蛋白水平。PRMT10的减少被AR或AR拮抗剂的下调所抵消。这些结果表明，PRMT10在雄激素依赖性前列腺癌细胞增殖中起重要作用。蛋白精氨酸甲基转移酶10在前列腺中的作用
Androgen receptor (AR) signaling is the master regulator of prostate cell growth. Here, to better understand AR signaling, we searched for AR-interacting proteins by yeast two-hybrid screening and identified protein arginine methyltransferase 10 (PRMT10) as one of the interacting proteins. PRMT10 was highly expressed in reproductive tissues, such as prostate. Immunostaining showed that PRMT10 was expressed in the nucleus of both epithelia and stroma of rat prostate. In human prostate cancer LNCaP cells, PRMT10 co-immunoprecipitated with AR in both the presence and absence of dihydrotestosterone (DHT). Knockdown of PRMT10 by siRNA decreased DHT-dependent LNCaP cell growth and induction of prostate-specific antigen, an AR-target gene, without apparent loss of AR. DHT decreased PRMT10 at both the mRNA and protein levels. The decrease in PRMT10 was canceled by knockdown of AR or an AR antagonist. These results indicate that PRMT10 plays an important role in androgen-dependent proliferation of prostate cancer cells. Graphical abstract Role of protein arginine methyltransferase 10 in prostate
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影响因子：
4.8
作者：
G. Prins;L. Birch
通讯作者：
G. Prins;L. Birch
影响因子：
4.8
作者：
Jiang, F;Wang, Z
通讯作者：
Wang, Z
雄激素受体：解开其独特反式激活结构域的秘密。
DOI：
10.1016/s0083-6729(05)71010-4
发表时间：
2005
期刊：
Vitamins and hormones.
影响因子：
--
作者：
Shen,HowardC;Coetzee,GerhardA
通讯作者：
Coetzee,GerhardA
影响因子：
56.9
作者：
CHANG, CS;KOKONTIS, J;LIAO, SS
通讯作者：
LIAO, SS