Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging.
Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging.
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DOI：
10.1038/s41467-022-27973-x
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发表时间：
2022-01-19
影响因子：
16.6
通讯作者：
Lin CP
中科院分区：
文献类型：
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作者：
Yeh SA;Hou J;Wu JW;Yu S;Zhang Y;Belfield KD;Camargo FD;Lin CP
造血干细胞的命运可以由包括细胞外钙离子浓度在内的微环境因素决定，但体内单个造血干细胞周围的局部钙离子浓度尚不清楚。在这里，我们开发了活体比率分析来量化小鼠颅骨骨髓中的绝对pH和[Ca~(2+)]e，考虑到钙探针的pH敏感性和通过骨骼的波长依赖的光学损失。出乎意料的是，骨髓中的平均[Ca~(2+)]e(1.0±0.5 4 mm)与血清中的[Ca~(2+)]e无显著差异，但HSC出现在局部[Ca~(2+)]e升高的部位(1.5±0.5 7 mm)。随着年龄的增长，仅支持激活的HSCs克隆性扩张的M型空洞中的[Ca~(2+)]e显著增加。因此，这项工作建立了一个高空间分辨率的工具来研究HSC壁龛中的[Ca~(2+)]e和pH，并可广泛应用于其他组织类型。造血干细胞的命运可以由钙离子浓度等因素控制。在这里，作者报告了一种活体比率分析方法，用于测量小鼠骨髓细胞外钙离子浓度和绝对pH。
The fate of hematopoietic stem cells (HSCs) can be directed by microenvironmental factors including extracellular calcium ion concentration ([Ca2+]e), but the local [Ca2+]e around individual HSCs in vivo remains unknown. Here we develop intravital ratiometric analyses to quantify the absolute pH and [Ca2+]e in the mouse calvarial bone marrow, taking into account the pH sensitivity of the calcium probe and the wavelength-dependent optical loss through bone. Unexpectedly, the mean [Ca2+]e in the bone marrow (1.0 ± 0.54 mM) is not significantly different from the blood serum, but the HSCs are found in locations with elevated local [Ca2+]e (1.5 ± 0.57 mM). With aging, a significant increase in [Ca2+]e is found in M-type cavities that exclusively support clonal expansion of activated HSCs. This work thus establishes a tool to investigate [Ca2+]e and pH in the HSC niche with high spatial resolution and can be broadly applied to other tissue types. The fate of hematopoietic stem cells can be controlled by factors such as calcium ion concentration. Here the authors report an intravital ratiometric analysis method to measure extracellular calcium ion concentrations and absolute pH in mouse bone marrow.
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影响因子：
14.5
作者：
LEENDERS, KL;PERANI, D;JONES, T
通讯作者：
JONES, T
影响因子：
64.8
作者：
Ohki, Kenichi;Chung, Sooyoung;Reid, R. Clay
通讯作者：
Reid, R. Clay
影响因子：
3.5
作者：
Lütcke H;Murayama M;Hahn T;Margolis DJ;Astori S;Zum Alten Borgloh SM;Göbel W;Yang Y;Tang W;Kügler S;Sprengel R;Nagai T;Miyawaki A;Larkum ME;Helmchen F;Hasan MT
通讯作者：
Hasan MT
影响因子：
3.4
作者：
Celli, A.;Sanchez, S.;Mauro, T.
通讯作者：
Mauro, T.
影响因子：
64.8
作者：
通讯作者：
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