Synthetic atrial natriuretic factors (ANFs) stimulate guanine 3',5'-monophosphate production but not hormone release in rat pituitary cells: peptide contamination with a gonadotropin-releasing hormone agonist explains luteinizing hormone-releasing activity of certain ANFs.
Synthetic atrial natriuretic factors (ANFs) stimulate guanine 3',5'-monophosphate production but not hormone release in rat pituitary cells: peptide contamination with a gonadotropin-releasing hormone agonist explains luteinizing hormone-releasing activity of certain ANFs.
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合成心房钠尿因子 (ANF) 刺激大鼠垂体细胞中鸟嘌呤 3,5-一磷酸的产生，但不刺激激素释放：促性腺激素释放激素激动剂的肽污染解释了某些 ANF 的黄体生成素释放活性。
DOI：
10.1210/endo-120-1-18
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发表时间：
1987
期刊：
影响因子：
4.8
通讯作者：
G. Aguilera
中科院分区：
文献类型：
--
作者：
A. Abou;K. Catt;G. Aguilera
研究了心房利钠因子（ANFs）对培养大鼠垂体细胞垂体前叶激素分泌和环核苷酸生成的影响。ANF对ACTH、GH、PRL和TSH的释放或cAMP的产生没有影响，无论是在基础激素水平上还是在适当的释放因子刺激它们的分泌时。然而，ANF显著刺激混合垂体前叶细胞和离心洗脱分离的富集垂体前叶细胞群中cGMP的产生。出乎意料的是，某些ANF制剂，Bachem大鼠ANF-(5-28)和大鼠ANF-(5-25)，显著刺激培养的垂体前叶细胞和富含促性腺激素的洗脱垂体细胞释放LH。同样的ANFs也取代了[125I-D-Lys6]GnRH乙胺与垂体前叶膜的结合，其释放h的活性与其相似。用特异性抗血清免疫沉淀ANF可消除ANF对cGMP产生的影响，但不改变ANF对LH释放的影响。综上所述，ANF不影响垂体前叶激素分泌和cAMP的产生，但刺激cGMP的形成。某些ANF制剂对LH释放的影响似乎可归因于肽污染与强效GnRH激动剂。
The effects of atrial natriuretic factors (ANFs) on anterior pituitary hormone secretion and cyclic nucleotide production were investigated in cultured rat pituitary cells. ANF had no effect on ACTH, GH, PRL, and TSH release or on cAMP production either on basal hormone levels or during stimulation of their secretion by the appropriate releasing factor. However, ANF markedly stimulated cGMP production in both mixed anterior pituitary cells and enriched anterior pituitary cell populations fractionated by centrifugal elutriation. Unexpectedly, certain ANF preparations, Bachem rat ANF-(5-28) and rat ANF-(5-25), markedly stimulated LH release from cultured anterior pituitary cells and gonadotroph-enriched elutriated pituitary cells. The same ANFs also displaced [125I-D-Lys6]GnRH ethylamide from binding to anterior pituitary membranes with potencies similar to their LH-releasing activities. Immunoprecipitation of ANF with a specific antiserum abolished the effect of ANF on cGMP production, but did not change the effect of ANF on LH release. In conclusion, ANF did not affect anterior pituitary hormone secretion or cAMP production, but stimulated cGMP formation. The effect of certain ANF preparations on LH release appears to be attributable to peptide contamination with a potent GnRH agonist.
大脑中的心房肽免疫反应性神经元：存在于心血管调节区域。
DOI：
10.1126/science.2858127
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Saper,CB;Standaert,DG;Currie,MG;Schwartz,D;Geller,DM;Needleman,P
通讯作者：
Needleman,P
大鼠下丘脑、心房和血浆中的心房钠尿因子：通过特异性放射免疫测定法测定。
DOI：
10.1016/0006-291x(84)91606-1
发表时间：
1984
影响因子：
3.1
作者：
Tanaka,I;Misono,KS;Inagami,T
通讯作者：
Inagami,T
鉴定假定的 GH 释放因子 (GRF) 批次主要为绵羊 CRF，并含有少量人 GRF。
影响因子：
4.8
作者：
Tannenbaum,GS;vanderRest,M;Downs,TR;Frohman,LA
通讯作者：
Frohman,LA