Functional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences.
Functional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences.
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DOI：
10.1186/gb-2014-15-6-r87
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发表时间：
2014-06-30
期刊：
影响因子：
12.3
通讯作者：
Hirt H
中科院分区：
文献类型：
--
作者：
Frei dit Frey N;Garcia AV;Bigeard J;Zaag R;Bueso E;Garmier M;Pateyron S;de Tauzia-Moreau ML;Brunaud V;Balzergue S;Colcombet J;Aubourg S;Martin-Magniette ML;Hirt H
丝裂原活化蛋白激酶（MAPK）是动植物免疫应答的关键调节因子。在拟南芥中，微生物相关分子模式（MAMPs）的感知激活了MPK3，MPK4和MPK6。越来越多的信息描绘了MAMPs在植物中激活的分子事件，但在这些信号事件中的MAPK的具体和合作的贡献在很大程度上是不清楚的。在这项工作中，我们分析了MPK3，MPK4和MPK6突变体在早期和晚期由细菌鞭毛蛋白的MAMP flg22引发的免疫应答中的行为。全基因组转录组分析显示，36%的flg22上调基因和68%的flg22下调基因在至少一种MAPK突变体中受到影响。MPK 4被认为是免疫的负调节因子，而MPK 3和MPK 6被认为在防御中发挥部分冗余的正功能。我们的工作表明，MPK4是需要调节约50%的flg22诱导的基因，我们确定了一个负作用MPK3在调节防御基因的表达，flg22诱导的水杨酸积累和抗病假单胞菌。在MAPK依赖性基因中，27%的flg22上调基因和76%的flg22下调基因需要两个或三个MAPK来调节。Flg22诱导的MAPK活性在MPK3和MPK6突变体中的幅度和持续时间均受到差异调节，揭示了高度相互依赖的网络。这些数据揭示了MPK3、MPK4和MPK6的一组新的不同功能，并表明植物免疫信号网络是通过这三种交织的MAPK通路的相互作用而编排的。
Mitogen-activated protein kinases (MAPKs) are key regulators of immune responses in animals and plants. In Arabidopsis, perception of microbe-associated molecular patterns (MAMPs) activates the MAPKs MPK3, MPK4 and MPK6. Increasing information depicts the molecular events activated by MAMPs in plants, but the specific and cooperative contributions of the MAPKs in these signalling events are largely unclear. In this work, we analyse the behaviour of MPK3, MPK4 and MPK6 mutants in early and late immune responses triggered by the MAMP flg22 from bacterial flagellin. A genome-wide transcriptome analysis reveals that 36% of the flg22-upregulated genes and 68% of the flg22-downregulated genes are affected in at least one MAPK mutant. So far MPK4 was considered as a negative regulator of immunity, whereas MPK3 and MPK6 were believed to play partially redundant positive functions in defence. Our work reveals that MPK4 is required for the regulation of approximately 50% of flg22-induced genes and we identify a negative role for MPK3 in regulating defence gene expression, flg22-induced salicylic acid accumulation and disease resistance to Pseudomonas syringae. Among the MAPK-dependent genes, 27% of flg22-upregulated genes and 76% of flg22-downregulated genes require two or three MAPKs for their regulation. The flg22-induced MAPK activities are differentially regulated in MPK3 and MPK6 mutants, both in amplitude and duration, revealing a highly interdependent network. These data reveal a new set of distinct functions for MPK3, MPK4 and MPK6 and indicate that the plant immune signalling network is choreographed through the interplay of these three interwoven MAPK pathways.
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影响因子：
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