The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
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DOI：
10.1016/j.molcel.2022.09.008
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发表时间：
2022-11-03
期刊：
影响因子：
16
通讯作者：
Corbett, Kevin D.
中科院分区：
文献类型：
--
作者：
Deep, Amar;Gu, Yajie;Gao, Yong-Qi;Ego, Kaori M.;Herzik Jr, Mark A.;Zhou, Huilin;Corbett, Kevin D.
自我与非自我的区分是整个生命中先天和适应性免疫的关键因素。在细菌中，CRISPR-Cas和限制修饰系统分别通过其序列和甲基化状态识别非自身核酸。在这里，我们表明，Wadjet防御系统识别DNA拓扑结构，以保护其宿主免受质粒转化。结合冷冻电子显微镜与交联质谱，我们表明，Wadjet形成一个复杂的类似的细菌凝聚素复合物MukBEF，与一个新的核酸酶亚基类似的II型DNA拓扑异构酶。Wadjet在需要SMC ATP酶亚基JetC水解ATP的反应中特异性切割闭环DNA，这表明该复合物可以使用DNA环挤出来感知其底物的拓扑结构，然后特异性激活核酸酶亚基JetD来切割质粒DNA。总的来说，我们的数据揭示了细菌如何通过拓扑传感来选择DNA维护机器来特异性识别和破坏外来DNA。细菌Wadjet防御系统保护其宿主细胞免受质粒转化。Deep等人结合生化分析和cryo-EM的结构测定表明，Wadjet通过将SMC家族DNA马达活性与同源二聚体核酸内切酶的特异性活化偶联来识别和切割闭合环状DNA。
Self versus non-self discrimination is a key element of innate and adaptive immunity across life. In bacteria, CRISPR-Cas and restriction-modification systems recognize non-self nucleic acids through their sequence and their methylation state, respectively. Here we show that the Wadjet defense system recognizes DNA topology to protect its host against plasmid transformation. Combining cryoelectron microscopy with crosslinking mass spectrometry, we show that Wadjet forms a complex similar to the bacterial condensin complex MukBEF, with a novel nuclease subunit similar to a type II DNA topoisomerase. Wadjet specifically cleaves closed-circular DNA in a reaction requiring ATP hydrolysis by the SMC ATPase subunit JetC, suggesting that the complex could use DNA loop extrusion to sense its substrate’s topology, then specifically activate the nuclease subunit JetD to cleave plasmid DNA. Overall, our data reveal how bacteria have co-opted a DNA maintenance machine to specifically recognize and destroy foreign DNAs through topology sensing. The bacterial Wadjet defense system protects its host cell from transformation by plasmids. Combining biochemical analysis and structure determination by cryo-EM, Deep et al. show that Wadjet recognizes and cleaves closed-circular DNA by coupling an SMC-family DNA motor activity to the specific activation of a homodimeric endonuclease.
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DOI：
10.1126/science.aar7831
发表时间：
2018-04-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ganji M;Shaltiel IA;Bisht S;Kim E;Kalichava A;Haering CH;Dekker C
通讯作者：
Dekker C
影响因子：
16
作者：
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Löwe J
DOI：
10.1146/annurev-cellbio-100814-125211
发表时间：
2015
影响因子：
11.3
作者：
Badrinarayanan A;Le TB;Laub MT
通讯作者：
Laub MT
DOI：
10.1126/science.aba0372
发表时间：
2020-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gao L;Altae-Tran H;Böhning F;Makarova KS;Segel M;Schmid-Burgk JL;Koob J;Wolf YI;Koonin EV;Zhang F
通讯作者：
Zhang F
影响因子：
16.6
作者：
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通讯作者：
Bramkamp, Marc