Identification and expression profile analysis of the sucrose phosphate synthase gene family in Litchi chinensis Sonn.
Identification and expression profile analysis of the sucrose phosphate synthase gene family in Litchi chinensis Sonn.
复制标题
荔枝蔗糖磷酸合酶基因家族的鉴定及表达谱分析
作者：
Wang D;Zhao J;Hu B;Li J;Qin Y;Chen L;Qin Y;Hu G
蔗糖磷酸合成酶（SPS，EC 2.4.1.14）是调节植物蔗糖生物合成的关键酶。SPS由不同的基因家族编码，表现出不同的表达模式和功能差异。SPS基因家族的全基因组鉴定和表达分析已在拟南芥、水稻和甘蔗中进行，但对荔枝SPS基因家族的全面分析尚不多见。目前还没有报道。本研究从荔枝中克隆了4个SPS基因（LcSPS 1、LcSPS 2、LcSPS 3和LcSPS 4）。基因组结构分析表明，4个荔枝SPS基因具有非常相似的外显子-内含子结构。系统进化树分析表明，LcSPS 1 -4分属于不同的SPS家族（LcSPS 1和LcSPS 2属于A家族，LcSPS 3属于B家族，LcSPS 4属于C家族）。LcSPS 1和LcSPS 4在花中强表达，而LcSPS 3在成熟叶片中表达最强。RT-qPCR结果表明，LcSPS基因在不同己糖/蔗糖比的品种假种皮发育过程中表达差异较大。LcSPS基因在五合里中表达量较高，成熟时其假种皮中蔗糖积累量较高。本研究揭示的LcSPS 1 -4基因在荔枝果实组织和发育阶段特异性表达增加了我们对这些基因在荔枝果实中所起重要作用的理解。
Sucrose phosphate synthase (SPS, EC 2.4.1.14) is a key enzyme that regulates sucrose biosynthesis in plants. SPS is encoded by different gene families which display differential expression patterns and functional divergence. Genome-wide identification and expression analyses of SPS gene families have been performed in Arabidopsis, rice, and sugarcane, but a comprehensive analysis of the SPS gene family in Litchi chinensis Sonn. has not yet been reported. In the current study, four SPS gene (LcSPS1, LcSPS2, LcSPS3, and LcSPS4) were isolated from litchi. The genomic organization analysis indicated the four litchi SPS genes have very similar exon-intron structures. Phylogenetic tree showed LcSPS1-4 were grouped into different SPS families (LcSPS1 and LcSPS2 in A family, LcSPS3 in B family, and LcSPS4 in C family). LcSPS1 and LcSPS4 were strongly expressed in the flowers, while LcSPS3 most expressed in mature leaves. RT-qPCR results showed that LcSPS genes expressed differentially during aril development between cultivars with different hexose/sucrose ratios. A higher level of expression of LcSPS genes was detected in Wuheli, which accumulates higher sucrose in the aril at mature. The tissue- and developmental stage-specific expression of LcSPS1-4 genes uncovered in this study increase our understanding of the important roles played by these genes in litchi fruits.
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