ACCT is a fast and accessible automatic cell counting tool using machine learning for 2D image segmentation.
ACCT is a fast and accessible automatic cell counting tool using machine learning for 2D image segmentation.
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DOI:
10.1038/s41598-023-34943-w
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发表时间:
2023-05-22
影响因子:
4.6
通讯作者:
中科院分区:
文献类型:
--
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Counting cells is a cornerstone of tracking disease progression in neuroscience. A common approach for this process is having trained researchers individually select and count cells within an image, which is not only difficult to standardize but also very time-consuming. While tools exist to automatically count cells in images, the accuracy and accessibility of such tools can be improved. Thus, we introduce a novel tool ACCT: Automatic Cell Counting with Trainable Weka Segmentation which allows for flexible automatic cell counting via object segmentation after user-driven training. ACCT is demonstrated with a comparative analysis of publicly available images of neurons and an in-house dataset of immunofluorescence-stained microglia cells. For comparison, both datasets were manually counted to demonstrate the applicability of ACCT as an accessible means to automatically quantify cells in a precise manner without the need for computing clusters or advanced data preparation.
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影响因子:
5.3
作者:
Karperien A;Ahammer H;Jelinek HF
通讯作者:
Jelinek HF
DOI:
10.1177/1073858414530512
发表时间:
2015-04
期刊:
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry
影响因子:
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通讯作者:
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通讯作者:
Oliphant TE
影响因子:
5.8
作者:
Arganda-Carreras, Ignacio;Kaynig, Verena;Seung, H. Sebastian
通讯作者:
Seung, H. Sebastian
DOI:
10.1016/j.isprsjprs.2020.01.013
发表时间:
2020-04-01
影响因子:
12.7
作者:
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通讯作者:
Wu, Chen