L-prostaglandin D synthase expression and regulation in mouse testis and epididymis during sexual maturation and testosterone treatment after castration
L-prostaglandin D synthase expression and regulation in mouse testis and epididymis during sexual maturation and testosterone treatment after castration
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性成熟过程中小鼠睾丸和附睾中 L-前列腺素 D 合酶的表达和调节以及去势后睾酮治疗
DOI：
10.1385/endo:24:1:039
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发表时间：
2004-06
期刊：
影响因子：
3.7
通讯作者：
Yang ZM.
中科院分区：
文献类型：
--
作者：
Zhu H;Ma H;Ni H;Ma XH;Mills N;Yang ZM.
脂质运载蛋白型前列腺素D合成酶（L-PGDS）在许多哺乳动物的睾丸和附睾中高度表达。本研究采用原位杂交和免疫组织化学方法，探讨L-PGDS在小鼠性成熟过程中睾丸和附睾中的表达，以及睾丸素一替代对L-PGDS在附睾中表达的影响。L-PGDS mRNA和蛋白在成年睾丸间质中均呈高表达。L-PGDS mRNA在生后30 d首次检测到，在成年附睾头和尾表达丰富。L-PGDS免疫染色在出生后第30天首次观察到。L-PGDS在成人附睾中呈强阳性表达。去势后3天，用溶媒或丙酸睾酮处理去势雄性小鼠。在对照组中，L-PGDS表达以时间依赖性方式稳步下降。对照组培养12 d，未见L-PGDS mRNA表达及免疫组化染色。丙酸睾丸酮处理5 d和12 d后，L-PGDS在整个附睾中的表达均显著增加。这些数据表明，L-PGDS在小鼠附睾中的表达逐渐下降，平行于去势后内源性雄激素浓度的下降，并增加与外源性睾酮的治疗，表明L-PGDS在小鼠附睾中的表达调节雄激素水平。然而，附睾不同区域的差异表达也可能受到来自睾丸的因子的影响。
Lipocalin-type prostaglandin D synthase (L-PGDS) is highly expressed in the adult testis and epididymis of many mammals. The present study was to investigate L-PGDS expression in mouse testis and epididymis during sexual maturation, and the effects of testoster-one replacement on L-PGDS expression in epididymis byin situhybridization and immunohistochemistry. Both L-PGDS mRNA and protein were highly expressed in the interstitial tissue of adult testis. L-PGDS mRNA was first detected on d 30 after birth and exhibited an abundant signal in adult caput and cauda epididymis. L-PGDS immunostaining was first observed on d 30 after birth. There was a strong level of L-PGDS immunostaining in adult epididymis. Castrated male mice were treated with either vehicle or testosterone propionate following 3 d postcastration. L-PGDS expression steadily declined in a time-dependent fashion in control groups. No L-PGDS mRNA expression or immunostaining was detected in the controls for 12 d. When the castrated mice were treated with testosterone propionate for 5 or 12 d, L-PGDS expression was significantly increased in the whole epididymis. These data suggest that L-PGDS expression in mouse epididymis gradually declined in parallel to the declining concentration of endogenous androgen after castration and increased with the treatment of exogenous testosterone, indicating that L-PGDS expression in mouse epididymis was modulated by androgen levels. However, differential expression in different areas of the epididymis may also be influenced by factors derived from the testis.
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影响因子：
3.6
作者：
S. Fouchécourt;F. Dacheux;J. Dacheux
通讯作者：
S. Fouchécourt;F. Dacheux;J. Dacheux
影响因子：
3.6
作者：
Y. Tokugawa;Ichiro Kunishige;Y. Kubota;K. Shimoya;T. Nobunaga;Tadashi Kimura;Fumitaka Saji;Yuji Murata;N. Eguchi;Hiroshi Oda;Y. Urade;O. Hayaishi
通讯作者：
Y. Tokugawa;Ichiro Kunishige;Y. Kubota;K. Shimoya;T. Nobunaga;Tadashi Kimura;Fumitaka Saji;Yuji Murata;N. Eguchi;Hiroshi Oda;Y. Urade;O. Hayaishi
影响因子：
64.5
作者：
Kanaoka, Y;Ago, H;Hayaishi, O
通讯作者：
Hayaishi, O
DOI：
10.1095/biolreprod66.2.524
发表时间：
2002-02
期刊：
--
影响因子：
--
作者：
S. Fouchécourt;P. Chaurand;B. DaGue;J. Lareyre;R. Matusik;R. Caprioli;M. Orgebin‐Crist
通讯作者：
S. Fouchécourt;P. Chaurand;B. DaGue;J. Lareyre;R. Matusik;R. Caprioli;M. Orgebin‐Crist
DOI：
10.1095/biolreprod.103.022079
发表时间：
2004-04
期刊：
--
影响因子：
--
作者：
Huiyang Zhu;Hong Ma;H. Ni;Xinghong Ma;N. Mills;Zeng-Ming Yang
通讯作者：
Huiyang Zhu;Hong Ma;H. Ni;Xinghong Ma;N. Mills;Zeng-Ming Yang