Hollow hydroxyapatite microspheres: a novel bioactive and osteoconductive carrier for controlled release of bone morphogenetic protein-2 in bone regeneration.
Hollow hydroxyapatite microspheres: a novel bioactive and osteoconductive carrier for controlled release of bone morphogenetic protein-2 in bone regeneration.
复制标题
中空羟基磷灰石微球：一种新型生物活性和骨传导载体，用于骨再生中骨形态发生蛋白2的控制释放。
DOI：
10.1016/j.actbio.2013.05.029
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发表时间：
2013-09
影响因子：
9.7
通讯作者：
Bal, B. Sonny
中科院分区：
文献类型：
--
作者：
Xiao, Wei;Fu, Hailuo;Rahaman, Mohamed N.;Liu, Yonxing;Bal, B. Sonny
关键词：
大面积骨缺损的修复是临床上常见而重要的问题。与现有治疗（如自体骨移植和同种异体骨移植）相关的局限性增加了对合成骨移植替代品的需求。本研究的目的是评价新型中空羟基磷灰石（HA）微球作为骨形态发生蛋白2（BMP 2）控释载体在骨再生中的能力。采用玻璃转化技术制备了具有高比表面积（> 100 m2/g）和介孔壳壁（孔径10 - 20 nm）的中空HA微球（106 - 150 μ m）。BMP 2从微球释放到由稀释的胎牛血清组成的培养基中在体外是缓慢的，但它持续发生超过2周。当植入大鼠颅骨缺损3或6周时，负载BMP 2的微球（1 μ g/缺损）显示出比不含BMP 2的微球更好的骨再生能力。在3周和6周时，植入BMP 2微球的缺损中的新骨量分别为40%和43%，而没有BMP 2的微球分别为13%和17%。用可生物降解的聚合物聚（乳酸-共-乙醇酸）涂覆载有BMP 2的微球，减少了体外释放的BMP 2的量，并且在一定的涂层厚度以上，显着减少了体内骨再生。结果表明，这种中空HA微球可以为骨再生中的生长因子提供一种具有生物活性和骨传导性的载体。
The regeneration of large bone defects is a common and significant clinical problem. Limitations associated with existing treatments such as autologous bone grafts and allografts have increased the need for synthetic bone graft substitutes. The objective of this study was to evaluate the capacity of novel hollow hydroxyapatite (HA) microspheres to serve as a carrier for controlled release of bone morphogenetic-2 (BMP2) in bone regeneration. Hollow HA microspheres (106–150 μm) with a high surface area (>100 m2/g) and a mesoporous shell wall (pore size 10–20 nm) were created using a glass conversion technique. The release of BMP2 from the microspheres into a medium composed of diluted fetal bovine serum in vitro was slow, but it occurred continuously for over 2 weeks. When implanted in rat calvarial defects for 3 or 6 weeks, the microspheres loaded with BMP2 (1 μg/defect) showed a significantly better capacity to regenerate bone than those without BMP2. The amount of new bone in the defects implanted with the BMP2-loaded microspheres was 40% and 43%, respectively, at 3 and 6 weeks, compared to 13% and 17%, respectively, for the microspheres without BMP2. Coating the BMP2-loaded microspheres with a biodegradable polymer, poly(lactic-co-glycolic acid), reduced the amount of BMP2 released in vitro and, above a certain coating thickness, significantly reduced bone regeneration in vivo. The results indicate that these hollow HA microspheres could provide a bioactive and osteoconductive carrier for growth factors in bone regeneration.
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DOI：
10.1007/s12306-008-0064-1
发表时间：
2008-12-01
期刊：
La Chirurgia degli organi di movimento
影响因子：
--
作者：
Devescovi, Valentina;Leonardi, Elisa;Cenni, Elisabetta
通讯作者：
Cenni, Elisabetta
影响因子：
4.9
作者：
Bi, Lianxiang;Jung, Steve;Bonewald, Lynda
通讯作者：
Bonewald, Lynda
影响因子：
4.2
作者：
Bonewald, LF;Harris, SE;Boskey, A
通讯作者：
Boskey, A
影响因子：
6.2
作者：
Feng, JQ;Zhang, JH;MacDougall, M
通讯作者：
MacDougall, M
DOI：
10.1016/j.msec.2013.01.048
发表时间：
2013-05-01
影响因子：
7.9
作者：
Fu, Hailuo;Rahaman, Mohamed N.;Brown, Roger F.;Day, Delbert E.
通讯作者：
Day, Delbert E.