Evaluation of BSA protein release from hollow hydroxyapatite microspheres into PEG hydrogel.
Evaluation of BSA protein release from hollow hydroxyapatite microspheres into PEG hydrogel.
复制标题
DOI：
10.1016/j.msec.2013.01.048
复制
发表时间：
2013-05-01
影响因子：
7.9
通讯作者：
Day, Delbert E.
中科院分区：
文献类型：
--
作者：
Fu, Hailuo;Rahaman, Mohamed N.;Brown, Roger F.;Day, Delbert E.
关键词：
同时作为骨传导基质和局部药物或生长因子输送装置的植入物可以为骨再生提供一个有吸引力的系统。在我们之前的工作中，我们制备了具有高比表面积、介孔壳壁的中空羟基磷灰石(简称HA)微球，并研究了模型蛋白牛血清白蛋白(BSA)从微球中释放到磷酸盐缓冲盐水(PBS)中的情况。本工作是我们以前工作的延伸，研究了BSA从类似的HA微球释放到生物相容的水凝胶--聚乙二醇凝胶中的情况。将负载牛血清白蛋白的HA微球置于聚乙二醇水溶液中，该溶液在紫外线照射下迅速凝胶化。用分光光度法测得BSA在聚乙二醇水凝胶中的释放速率比在PBS中的释放速度慢，这取决于初始的BSA负载量和微球壳壁的微观结构。最初加载到微球中的总共35-40%的BSA在~14天内被释放到聚乙二醇中。结果表明，这些中空的HA微球在骨再生和骨疾病的治疗中具有作为局部药物或生长因子输送的骨传导装置的潜在潜力。
Implants that simultaneously function as an osteoconductive matrix and as a device for local drug or growth factor delivery could provide an attractive system for bone regeneration. In our previous work, we prepared hollow hydroxyapatite (abbreviated HA) microspheres with a high surface area, mesoporous shell wall and studied the release of a model protein, bovine serum albumin (BSA), from the microspheres into phosphate-buffered saline (PBS). The present work is an extension of our previous work to study the release of BSA from similar HA microspheres into a biocompatible hydrogel, poly(ethylene glycol) (PEG). BSA-loaded HA microspheres were placed in a PEG solution which was rapidly gelled using ultraviolet radiation. The BSA release rate into the PEG hydrogel, measured using a spectrophotometric method, was slower than into PBS, and it was dependent on the initial BSA loading and on the microstructure of the microsphere shell wall. A total of 35–40% of the BSA initially loaded into the microspheres was released into PEG over ~14 days. The results indicate that these hollow HA microspheres have promising potential as an osteoconductive device for local drug or growth factor delivery in bone regeneration and in the treatment of bone diseases.
登录
查看更多内容
DOI：
10.1016/j.bioeng.2007.08.001
发表时间：
2007-11-01
期刊：
BIOMOLECULAR ENGINEERING
影响因子：
--
作者：
Chai, F.;Hornez, J. -C.;Hildebrand, H. F.
通讯作者：
Hildebrand, H. F.
影响因子：
3.6
作者：
ONO, I;OHURA, T;KUBOKI, Y
通讯作者：
KUBOKI, Y
影响因子：
3.6
作者：
RIPAMONTI, U;MA, SS;REDDI, AH
通讯作者：
REDDI, AH
影响因子：
3
作者：
LUSTIG, SR;PEPPAS, NA
通讯作者：
PEPPAS, NA
DOI：
10.1097/01.blo.0000175713.30506.77
发表时间：
2005-08-01
影响因子：
4.2
作者：
Hanssen, AD
通讯作者：
Hanssen, AD