Comparative genomics of mutualistic viruses of Glyptapanteles parasitic wasps.
Comparative genomics of mutualistic viruses of Glyptapanteles parasitic wasps.
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DOI：
10.1186/gb-2008-9-12-r183
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发表时间：
2008
期刊：
影响因子：
12.3
通讯作者：
Nene, Vishvanath
中科院分区：
文献类型：
--
作者：
Desjardins, Christopher A.;Gundersen-Rindal, Dawn E.;Hostetler, Jessica B.;Tallon, Luke J.;Fadrosh, Douglas W.;Fuester, Roger W.;Pedroni, Monica J.;Haas, Brian J.;Schatz, Michael C.;Jones, Kristine M.;Crabtree, Jonathan;Forberger, Heather;Nene, Vishvanath
两个内共生多脱氧核糖核酸病毒的比较基因组分析Glyptapanteles寄生蜂揭示了新的见解主机和寄生虫之间的进化军备竞赛。多DNA病毒是一种基因组分段的双链DNA病毒，是寄生蜂的专性内共生体。病毒颗粒是复制缺陷型的，由雌性黄蜂从整合到黄蜂基因组中的前病毒序列产生。这些颗粒与卵共同注射到毛虫宿主中，在那里病毒基因表达促进寄生虫存活，从而促进前病毒DNA的存活。在这里，我们的特点和比较多核糖核酸病毒科与舞毒蛾寄生蜂，沿着与近完整的前病毒序列，这两个病毒基因组的衍生。Glyptapanteles indiensis和Glyptapanteles flavicoxis的杆状病毒基因组由29个不同大小的片段组成，分别为517和594 kbp。它们由黄蜂基因组中至少7个不同的基因座产生。这些序列的注释揭示了多DNA病毒的许多新特征，包括昆虫样糖转运蛋白基因和转座因子。进化分析表明，正选择是广泛存在于病毒基因。G. indiensis和G.已确认黄氧化症臂病毒前病毒片段是包含一个到多个病毒片段的多个基因座，侧翼由黄蜂基因编码DNA隔开。杆状病毒基因的快速进化支持杆状病毒基因在杆状病毒和毛虫之间的“军备竞赛”的假设。编码糖转运蛋白的杆状病毒基因的系统发育分析为一些多DNA病毒基因的黄蜂起源提供了第一个有力的证据。我们假设转座因子，如这里描述的那些，可以促进基因在前病毒片段和宿主DNA之间的转移。
Comparative genome analysis of two endosymbiotic polydnaviruses from Glyptapanteles parasitic wasps reveals new insights into the evolutionary arms race between host and parasite. Polydnaviruses, double-stranded DNA viruses with segmented genomes, have evolved as obligate endosymbionts of parasitoid wasps. Virus particles are replication deficient and produced by female wasps from proviral sequences integrated into the wasp genome. These particles are co-injected with eggs into caterpillar hosts, where viral gene expression facilitates parasitoid survival and, thereby, survival of proviral DNA. Here we characterize and compare the encapsidated viral genome sequences of bracoviruses in the family Polydnaviridae associated with Glyptapanteles gypsy moth parasitoids, along with near complete proviral sequences from which both viral genomes are derived. The encapsidated Glyptapanteles indiensis and Glyptapanteles flavicoxis bracoviral genomes, each composed of 29 different size segments, total approximately 517 and 594 kbp, respectively. They are generated from a minimum of seven distinct loci in the wasp genome. Annotation of these sequences revealed numerous novel features for polydnaviruses, including insect-like sugar transporter genes and transposable elements. Evolutionary analyses suggest that positive selection is widespread among bracoviral genes. The structure and organization of G. indiensis and G. flavicoxis bracovirus proviral segments as multiple loci containing one to many viral segments, flanked and separated by wasp gene-encoding DNA, is confirmed. Rapid evolution of bracovirus genes supports the hypothesis of bracovirus genes in an 'arms race' between bracovirus and caterpillar. Phylogenetic analyses of the bracoviral genes encoding sugar transporters provides the first robust evidence of a wasp origin for some polydnavirus genes. We hypothesize transposable elements, such as those described here, could facilitate transfer of genes between proviral segments and host DNA.
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