Fast pixel shifting phase unwrapping algorithm in quantitative interferometric microscopy

Fast pixel shifting phase unwrapping algorithm in quantitative interferometric microscopy
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定量干涉显微镜中的快速像素平移相位展开算法

DOI:
10.1117/12.2070969
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发表时间:
2014-06
期刊:
Chin. Opt. Lett.
影响因子:
--
通讯作者:
Fei Liu
Fei Liu
中科院分区:
其他
文献类型:
--
作者:
Liang Xue;Shouyu Wang;Keding Yan;Nan Sun;Zhenhua Li;Fei Liu

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定量干涉显微镜是观察生物样品(例如细胞和组织)的重要方法。为了从干涉图中获得样品的连续相分布,相位提取和相位分布是bot
Quantitative interferometric microscopy is an important method for observing biological samples such as cells and tissues. In order to obtain continuous phase distribution of the sample from the interferogram, phase extracting and phase unwrapping are both needed in quantitative interferometric microscopy. Phase extracting includes fast Fourier transform method and Hilbert transform method, etc., almost all of them are rapid methods. However, traditional unwrapping methods such as least squares algorithm, minimum network flow method, etc. are time-consuming to locate the phase discontinuities which lead to low processing efficiency. Other proposed high-speed phase unwrapping methods always need at least two interferograms to recover final phase distributions which cannot realize real time processing. Therefore, high-speed phase unwrapping algorithm for single interferogram is required to improve the calculation efficiency. Here, we propose a fast phase unwrapping algorithm to realize high-speed quantitative interferometric microscopy, by shifting mod 2π wrapped phase map for one pixel, then multiplying the original phase map and the shifted one, then the phase discontinuities location can be easily determined. Both numerical simulation and experiments confirm that the algorithm features fast, precise and reliable.
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