Isoflurane preconditioning improves short-term and long-term neurological outcome after focal brain ischemia in adult rats.
Isoflurane preconditioning improves short-term and long-term neurological outcome after focal brain ischemia in adult rats.
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DOI：
10.1016/j.neuroscience.2009.08.011
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发表时间：
2009-12-01
期刊：
影响因子：
3.3
通讯作者：
Zuo, Z.
中科院分区：
文献类型：
--
作者：
Li, L.;Zuo, Z.
异氟醚预适应改善成年大鼠局灶性脑缺血后的短期神经预后。目前尚不清楚地氟醚是否导致脑内预适应的延迟阶段，以及异氟醚预适应诱导的神经保护是否长期存在。2月龄SD雄性大鼠暴露于或不暴露于异氟醚或地氟烷30min后，于麻醉后24小时行大脑中动脉阻断(MCAO)90min。在MCAO后24小时或4周评估神经学结果。于MCAO后4周检测半影区大脑皮质末端脱氧核苷酸转移酶生物素标记缺口末端标记物(TUNEL)阳性细胞密度。同时给予异氟醚或地氟醚预处理30min。24小时后取脑皮质，定量检测B细胞淋巴瘤-2的表达。在这里，我们发现，在MCAO后24小时，使用1.1%或2.2%的异氟醚，而不是6%或12%的地氟烷，可增加大脑皮层B细胞淋巴瘤-2的表达，改善神经功能，缩小脑梗塞体积。异氟醚预适应还改善了大脑中动脉阻塞4周后评估的大鼠的神经功能并减少了脑梗塞体积。异氟醚预处理还可降低半影区大脑皮层TUNEL阳性细胞密度。我们的结论是，异氟醚预适应改善了成年大鼠短暂性局灶性脑缺血后的短期和长期神经学结果，并减少了延迟性细胞死亡。Bcl2可能参与了异氟醚的预适应作用。地氟醚预处理不会导致延迟的神经保护阶段。
Isoflurane preconditioning improved short-term neurological outcome after focal brain ischemia in adult rats. It is not known whether desflurane induces a delayed phase of preconditioning in the brain and whether isoflurane preconditioning-induced neuroprotection is long-lasting. Two-month-old Sprague-Dawley male rats were exposed to or were not exposed to isoflurane or desflurane for 30 min and then subjected to a 90-min middle cerebral arterial occlusion (MCAO) at 24 hr after the anesthetic exposure. Neurological outcome was evaluated at 24 hr or 4 weeks after the MCAO. The density of the terminal deoxynucleotidyl transferase biotinylated UTP nick end labeling (TUNEL) positive cells in the penumbral cerebral cortex were assessed 4 weeks after the MCAO. Also, rats were pretreated with isoflurane or desflurane for 30 min. Their cerebral cortices were harvested for quantifying B-cell lymphoma-2 expression 24 hr later. Here, we showed that pretreatment with 1.1% or 2.2% isoflurane, but not with 6% or 12% desflurane, increased B-cell lymphoma-2 expression in the cerebral cortex, improved neurological functions and reduced infarct volumes evaluated at 24 hr after the MCAO. Isoflurane preconditioning also improved neurological functions and reduced brain infarct volumes in rats evaluated 4 weeks after the MCAO. Isoflurane preconditioning also decreased the density of TUNEL-positive cells in the penumbral cerebral cortex. We conclude that isoflurane preconditioning improves short-term and long-term neurological outcome and reduces delayed cell death after transient focal brain ischemia in adult rats. Bcl-2 may be involved in the isoflurane preconditioning effect. Desflurane pretreatment did not induce a delayed phase of neuroprotection.
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