Evaluation of the role of Candida albicans agglutinin-like sequence (Als) proteins in human oral epithelial cell interactions.
Evaluation of the role of Candida albicans agglutinin-like sequence (Als) proteins in human oral epithelial cell interactions.
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DOI：
10.1371/journal.pone.0033362
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Naglik JR
中科院分区：
文献类型：
--
作者：
Murciano C;Moyes DL;Runglall M;Tobouti P;Islam A;Hoyer LL;Naglik JR
真菌白色念珠菌在定植和发病过程中使用粘附素与人上皮表面相互作用。白念珠菌ALS（凝集素样序列）基因家族编码8个具有粘附功能的大细胞表面糖蛋白（Als1-Als7和Als9）。本研究利用白色念珠菌Δals突变株研究了Als家族在口腔上皮细胞粘附和损伤、细胞因子诱导以及基于map1 （MKP1/c-Fos）的区分酵母和菌丝的信号通路激活中的作用。在测试的8个Δals突变株中，只有Δals3菌株显示出口腔上皮细胞粘附和损伤以及细胞因子产生的显著减少。高真菌：感染的上皮细胞多样性能够挽救细胞损伤和细胞因子产生表型，证明真菌负担在粘膜感染中的重要性。尽管存在粘附、损伤和细胞因子诱导表型，Δals3菌株诱导MKP1磷酸化和c-Fos产生的程度与对照细胞相似。我们的数据表明，Als3直接参与上皮粘附，但间接参与细胞损伤和细胞因子诱导，并且不是口腔上皮细胞区分酵母和菌丝形式的白色念珠菌的靶向因子。
The fungus C. albicans uses adhesins to interact with human epithelial surfaces in the processes of colonization and pathogenesis. The C. albicans ALS (agglutinin-like sequence) gene family encodes eight large cell-surface glycoproteins (Als1-Als7 and Als9) that have adhesive function. This study utilized C. albicans Δals mutant strains to investigate the role of the Als family in oral epithelial cell adhesion and damage, cytokine induction and activation of a MAPK-based (MKP1/c-Fos) signaling pathway that discriminates between yeast and hyphae. Of the eight Δals mutants tested, only the Δals3 strain showed significant reductions in oral epithelial cell adhesion and damage, and cytokine production. High fungal:epithelial cell multiplicities of infection were able to rescue the cell damage and cytokine production phenotypes, demonstrating the importance of fungal burden in mucosal infections. Despite its adhesion, damage and cytokine induction phenotypes, the Δals3 strain induced MKP1 phosphorylation and c-Fos production to a similar extent as control cells. Our data demonstrate that Als3 is involved directly in epithelial adhesion but indirectly in cell damage and cytokine induction, and is not the factor targeted by oral epithelial cells to discriminate between the yeast and hyphal form of C. albicans.
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口腔上皮细胞中MAPK/c-FOS诱导反应的激活特异于白色念珠菌和都柏林菌丝菌丝。
影响因子：
5.4
作者：
Moyes DL;Murciano C;Runglall M;Kohli A;Islam A;Naglik JR
通讯作者：
Naglik JR
影响因子：
30.3
作者：
Moyes DL;Runglall M;Murciano C;Shen C;Nayar D;Thavaraj S;Kohli A;Islam A;Mora-Montes H;Challacombe SJ;Naglik JR
通讯作者：
Naglik JR
影响因子：
2.5
作者：
Hoyer, LL;Payne, TL;Scherer, S
通讯作者：
Scherer, S
DOI：
10.1016/s1357-2725(98)00081-8
发表时间：
1998-10-01
影响因子：
4
作者：
Stylianou, E;Saklatvala, J
通讯作者：
Saklatvala, J
影响因子：
2.9
作者：
Hoyer, Lois L.;Green, Clayton B.;Zhao, Xiaomin
通讯作者：
Zhao, Xiaomin