Correlation between intestinal flora disruption and protein-energy wasting in patients with end-stage renal disease.
Correlation between intestinal flora disruption and protein-energy wasting in patients with end-stage renal disease.
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DOI：
10.1186/s12882-022-02762-2
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发表时间：
2022-04-04
期刊：
影响因子：
2.3
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Hu J;Zhong X;Liu Y;Yan J;Zhou D;Qin D;Xiao X;Zheng Y;Wen L;Tan R;Liang P;Liu Y
不同的透析治疗可能会影响透析治疗的慢性肾脏病（CKD）患者肠道植物群的组成和结构。本研究旨在分析不同植物群与营养指标之间的相关性，进一步探讨终末期肾病（ESRD）CKD患者的潜在代谢途径。共招募了102例ESRD患者，并将其分为血液透析（HD）组（N = 49）和腹膜透析（PD）组（N = 53）。测定其生化指标、人体测量学指标和炎症标志物。提取总基因组DNA进行16 S核糖体DNA测序。并利用生物信息学方法进行功能分析。HD组和PD组的人体测量指标（包括握力、中上臂围、中上臂肌围和体重指数）与产丁酸细菌（Rosella和Phascolarctobacterium）呈正相关，与条件致病菌（埃希氏菌属）呈负相关。同时，高敏C反应蛋白和白细胞介素6等炎症标志物在PD-蛋白质-能量消耗（PEW）组显著高于PD-非蛋白质-能量消耗（NPEW）组; HD-PEW组虽有升高趋势，但无显著差异。玫瑰茄在HD-PEW组中比在HD-NPEW组中相当稀少，而埃希氏菌在PD-PEW组中比在PD-NPEW组中显著更丰富。与HD组相比，PD组的必需氨基酸合成途径、氨基酸代谢相关酶途径和氨酰转运RNA生物合成途径减弱。大多数碳水化合物代谢途径减弱，虽然三羧酸循环略有增强。同时，脂肪酸代谢增强，脂肪酸合成减弱; B族维生素的代谢途径也减弱。肠道植物群释放的各种化合物的这些潜在代谢途径与血液生化指标、人体测量指标和炎症标志物显示出显著的相关性。在ESRD患者中，不同的透析治疗影响了产丁酸细菌（Rosella和Phascolarctobacterium）和条件致病菌（埃希氏菌属）的丰度。丁酸产生菌与PEW呈正相关，与大肠杆菌呈负相关。改善肠道多样性和增加产丁酸细菌（如Blautella，Faecococcus和Phascolarctobacterium）的数量是提高ESRD患者蛋白质能量消耗的潜在治疗方法。
Different dialysis treatments may affect the composition and structure of the intestinal flora of dialysis-treated chronic kidney disease (CKD) patients. This study aimed to analyze the correlations between the different flora and the nutritional indexes and further explore the potential metabolic pathways in patients with CKD in end-stage renal disease (ESRD). Altogether, 102 patients with ESRD were recruited and categorized into the hemodialysis (HD) group (N = 49) and the peritoneal dialysis (PD) group (N = 53). Their biochemical indexes, anthropometric indicators, and inflammatory markers were determined. The total genomic DNA was extracted for 16S ribosomal DNA sequencing. Furthermore, bioinformatics analysis was employed for functional analysis. Anthropometric indicators, including handgrip strength, mid-upper arm circumference, mid-upper arm muscle circumference, and body mass index, in the HD and PD groups showed a positive correlation with butyric acid-producing bacteria (Rosella and Phascolarctobacterium) and a negative correlation with conditional pathogens (Escherichia spp.). Meanwhile, the inflammatory markers, including high-sensitivity C-reactive protein and interleukin-6, were significantly higher in the PD-protein–energy wasting (PEW) group than in the PD-non-protein–energy wasting (NPEW) group; although they showed an increasing trend in the HD-PEW group, no significant difference was noted. Rosella was considerably scarce in the HD-PEW group than in the HD-NPEW group, whereas Escherichia was substantially more abundant in the PD-PEW group than in the PD-NPEW group. Compared with the HD group, the essential amino acid synthesis pathway, amino acid metabolism-related enzyme pathways, and aminoacyl-transfer RNA biosynthesis pathways were weakened in the PD group. Most carbohydrate metabolic pathways were weakened, although the tricarboxylic acid cycle was slightly enhanced. Concurrently, the fatty acid metabolism was enhanced, whereas fatty acid synthesis was weakened; the metabolic pathways of B vitamins were also weakened. These potential metabolic pathways of the various compounds released by intestinal flora showed a significant correlation with blood biochemical indexes, anthropometric indicators, and inflammatory markers. In patients with ESRD, different dialysis treatments affected the abundance of butyric acid-producing bacteria (Rosella and Phascolarctobacterium) and conditional pathogens (Escherichia spp.). Butyric acid-producing bacteria showed a positive correlation with PEW and showed a negative correlation with Escherichia. Improving the intestinal diversity and increasing the amount of butyric acid-producing bacteria, such as Blautella, Faecococcus, and Phascolarctobacterium, are potential therapeutic approaches to enhance protein–energy consumption in patients with ESRD.
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影响因子：
14.9
作者：
Kanehisa M;Furumichi M;Sato Y;Ishiguro-Watanabe M;Tanabe M
通讯作者：
Tanabe M
影响因子：
4.6
作者：
Jang HM;Lee KE;Lee HJ;Kim DH
通讯作者：
Kim DH
影响因子：
5.9
作者：
Kang SS;Chang JW;Park Y
通讯作者：
Park Y
影响因子：
3.4
作者：
Davis, Cassie Jo;Sowa, Diane;Peterson, Sarah
通讯作者：
Peterson, Sarah
DOI：
10.1126/science.1229000
发表时间：
2013-02-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Smith MI;Yatsunenko T;Manary MJ;Trehan I;Mkakosya R;Cheng J;Kau AL;Rich SS;Concannon P;Mychaleckyj JC;Liu J;Houpt E;Li JV;Holmes E;Nicholson J;Knights D;Ursell LK;Knight R;Gordon JI
通讯作者：
Gordon JI