New Insight into the Role of the Calvin Cycle: Reutilization of CO2 Emitted through Sugar Degradation.
New Insight into the Role of the Calvin Cycle: Reutilization of CO2 Emitted through Sugar Degradation.
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DOI：
10.1038/srep11617
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发表时间：
2015-07-01
影响因子：
4.6
通讯作者：
Fukui T
中科院分区：
文献类型：
--
作者：
Shimizu R;Dempo Y;Nakayama Y;Nakamura S;Bamba T;Fukusaki E;Fukui T
Ralstonia eutropha是一种兼性化能石自养细菌，利用Calvin-Benson-Bassham （CBB）循环固定CO2。本研究表明，真核菌H16G将[1-13C1]-葡萄糖氧化脱羧释放的13CO2转化为CBB循环的关键代谢物，最终转化为13C丰度高达5.6%的聚（3-羟基丁酸盐）[P(3HB)]。菌株H16G从葡萄糖产生的P（3HB）的碳产量比CBB循环失活突变体高1.2倍，这与通过糖降解与CBB循环结合的能量平衡和还原性当量估计的二氧化碳固定可能一致。结果证明，在好氧异养条件下，富营养菌的“无偿”功能CBB循环参与了糖降解过程中排放的CO2的再利用，其优势表现为储存化合物的碳产量增加。这是对CBB循环作用的新认识，可能适用于更有效地利用生物质资源。
Ralstonia eutropha is a facultative chemolithoautotrophic bacterium that uses the Calvin–Benson–Bassham (CBB) cycle for CO2 fixation. This study showed that R. eutropha strain H16G incorporated 13CO2, emitted by the oxidative decarboxylation of [1-13C1]-glucose, into key metabolites of the CBB cycle and finally into poly(3-hydroxybutyrate) [P(3HB)] with up to 5.6% 13C abundance. The carbon yield of P(3HB) produced from glucose by the strain H16G was 1.2 times higher than that by the CBB cycle-inactivated mutants, in agreement with the possible fixation of CO2 estimated from the balance of energy and reducing equivalents through sugar degradation integrated with the CBB cycle. The results proved that the ‘gratuitously’ functional CBB cycle in R. eutropha under aerobic heterotrophic conditions participated in the reutilization of CO2 emitted during sugar degradation, leading to an advantage expressed as increased carbon yield of the storage compound. This is a new insight into the role of the CBB cycle, and may be applicable for more efficient utilization of biomass resources.
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影响因子：
64.5
作者：
Clasquin MF;Melamud E;Singer A;Gooding JR;Xu X;Dong A;Cui H;Campagna SR;Savchenko A;Yakunin AF;Rabinowitz JD;Caudy AA
通讯作者：
Caudy AA
影响因子：
5
作者：
Kato, M;Bao, HJ;Doi, Y
通讯作者：
Doi, Y
影响因子：
4.1
作者：
Dempo Y;Ohta E;Nakayama Y;Bamba T;Fukusaki E
通讯作者：
Fukusaki E
影响因子：
4.4
作者：
Brigham, Christopher J.;Speth, Daan R.;Sinskey, Anthony J.
通讯作者：
Sinskey, Anthony J.
影响因子：
2.8
作者：
Chinen, Akito;Kozlov, Yuri I.;Yasueda, Hisashi
通讯作者：
Yasueda, Hisashi