CCL22 to Activate Treg Migration and Suppress Depigmentation in Vitiligo.
CCL22 to Activate Treg Migration and Suppress Depigmentation in Vitiligo.
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DOI：
10.1038/jid.2015.26
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发表时间：
2015-06
影响因子：
6.5
通讯作者：
Le Poole, I. Caroline
中科院分区：
文献类型：
--
作者：
Eby, Jonathan M.;Kang, Hee-Kap;Tully, Sean T.;Bindeman, Wendy E.;Peiffer, Daniel S.;Chatterjee, Shilpak;Mehrotra, Shikhar;Le Poole, I. Caroline
在白癜风中，针对黑色素细胞的皮肤部署和细胞毒性T细胞活性伴随着白癜风患者皮肤中的调节性T细胞（Treg），表明自身免疫反应并不能充分地固定在检查中。我们假设CCL22的表达能够促进treg皮肤抑制小鼠CCL22基因。将克隆到表达载体中，并将其用于基因枪处理。分开，每周进行扫描，并监测相关T细胞的激活和增殖，并显着降低了皮肤治疗后2周，通过在皮肤中明显增加了Treg的抽象，以黑素细胞反应性，TCR转基因T细胞以及降低增殖和减少IFN-γ的产生，以响应Cognate持续治疗。局部免疫抑制所必需的。
In vitiligo, gradual cutaneous depigmentation and cytotoxic T cell activity against melanocytes is accompanied by a paucity of regulatory T cells (Tregs) in vitiligo patient skin, indicating that autoimmune responses are not adequately held in check. Thus we sought a means to repopulate patient skin with Tregs. We hypothesized that enhanced expression of CCL22 can promote Treg skin homing to suppress depigmentation. The mouse Ccl22 gene was cloned into an expression vector and resulting DNA was used for gene gun treatment. Two spontaneous depigmentation models with different kinetics of melanocyte loss were utilized, expressing tyrosinase-reactive and gp100-reactive T cell receptor transgenes. Mice were subjected to 5 gene gun treatments 6 days apart, scanned for depigmentation weekly thereafter and monitored for activation and proliferation of relevant T cells and for Treg infiltration to the skin. Significantly reduced depigmentation 2 weeks after treatment was accompanied by a markedly increased abundance of Tregs in the skin at the expense of melanocyte reactive, TCR transgenic T cells as well as by reduced proliferation and reduced IFN-γ production in response to cognate peptide. Continued treatment may be necessary for sustained, local immunosuppression. These findings suggest that topical CCL22 may be used for the treatment of vitiligo.
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影响因子：
4.6
作者：
Moriyama M;Hayashida JN;Toyoshima T;Ohyama Y;Shinozaki S;Tanaka A;Maehara T;Nakamura S
通讯作者：
Nakamura S
影响因子：
5.1
作者：
Butti, E.;Bergami, A.;Furlan, R.
通讯作者：
Furlan, R.
影响因子：
3.7
作者：
Hoffmann, Andreas;Kovermann, Michael;Pfeifer, Sven
通讯作者：
Pfeifer, Sven
影响因子：
6.5
作者：
Denman, Cecele J.;McCracken, James;Le Poole, I. Caroline
通讯作者：
Le Poole, I. Caroline
影响因子：
2.2
作者：
Ekman, B.;Alstrand, N.;Wahlberg, J.
通讯作者：
Wahlberg, J.