Cytokine/chemokine profiles contribute to understanding the pathogenesis and diagnosis of primary Sjögren's syndrome.
Cytokine/chemokine profiles contribute to understanding the pathogenesis and diagnosis of primary Sjögren's syndrome.
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DOI：
10.1111/j.1365-2249.2012.04587.x
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发表时间：
2012-07
影响因子：
4.6
通讯作者：
Nakamura S
中科院分区：
文献类型：
--
作者：
Moriyama M;Hayashida JN;Toyoshima T;Ohyama Y;Shinozaki S;Tanaka A;Maehara T;Nakamura S
通过检测自身免疫性损伤部位细胞因子、趋化因子及趋化因子受体的表达模式，探讨干燥综合征（SS）局部自身免疫性损伤的发病机制。采用实时荧光定量聚合酶链反应方法检测了36例SS患者唇唾液腺（LSGs）和外周血单个核细胞（PBMC）中这些分子的mRNA表达。免疫组化法检测LSG标本中浸润淋巴细胞亚群和趋化因子/趋化因子受体的表达。使用流式细胞术或酶联免疫吸附测定法分析唾液中的细胞因子/趋化因子浓度。辅助性T细胞1型（Th 1）细胞因子、趋化因子和趋化因子受体的mRNA表达在LSG中高于PBMC。相反，Th 2细胞因子、趋化因子[胸腺和活化调节趋化因子（TARC/CCL 17）、巨噬细胞衍生趋化因子（MDC/CCL 22）]和趋化因子受体（CCR 4）的mRNA表达与LSG中的强淋巴细胞蓄积密切相关。此外，TARC和MDC在LSG中的导管上皮细胞中/周围被化学检测到，而CCR 4在浸润的淋巴细胞上被检测到。这些细胞因子/趋化因子的浓度在SS患者的唾液中显著高于对照组，并且Th 2细胞因子/趋化因子的浓度与LSG中的强淋巴细胞蓄积密切相关。这些结果表明，SS可能启动和/或维持的Th 1和Th 17细胞和进展与Th 2细胞通过特定的趋化因子/趋化因子受体之间的相互作用。此外，唾液中的细胞因子/趋化因子的测量被认为是有用的诊断，也揭示疾病状态。
To investigate the pathogenesis of localized autoimmune damage in Sjögren's syndrome (SS) by examining the expression patterns of cytokines, chemokines and chemokine receptors at sites of autoimmune damage. mRNA expression of these molecules in the labial salivary glands (LSGs) and peripheral blood mononuclear cells (PBMCs) from 36 SS patients was examined using a real-time polymerase chain reaction-based method. Subsets of the infiltrating lymphocytes and chemokines/chemokine receptors expression in the LSG specimens were examined by immunohistochemistry. Cytokines/chemokine concentrations in the saliva were analysed using flow cytometry or enzyme-linked immunosorbent assay. mRNA expression of T helper type 1 (Th1) cytokines, chemokines and chemokine receptors was higher in LSGs than in PBMCs. In contrast, mRNA expression of Th2 cytokines, chemokines [thymus and activation-regulated chemokine (TARC/CCL17), macrophage-derived chemokine (MDC/CCL22)] and chemokine receptor (CCR4) was associated closely with strong lymphocytic accumulation in LSGs. Furthermore, TARC and MDC were detected immunohistochemically in/around the ductal epithelial cells in LSGs, whereas CCR4 was detected on infiltrating lymphocytes. The concentrations of these cytokines/chemokines were significantly higher in the saliva from SS patients than those from controls, and the concentrations of Th2 cytokines/chemokines were associated closely with strong lymphocytic accumulation in LSGs. These results suggest that SS might be initiated and/or maintained by Th1 and Th17 cells and progress in association with Th2 cells via the interaction between particular chemokines/chemokine receptors. Furthermore, the measurement of cytokines/chemokines in saliva is suggested to be useful for diagnosis and also to reveal disease status.
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DOI：
10.1016/0030-4220(74)90417-4
发表时间：
1974-01-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
GREENSPAN, JS;DANIELS, TE;SYLVESTER, RA
通讯作者：
SYLVESTER, RA
影响因子：
15.9
作者：
Haskell, CA;Hancock, WW;Charo, IF
通讯作者：
Charo, IF
影响因子：
--
作者：
Ohyama, Y;Nakamura, S;Nomoto, K
通讯作者：
Nomoto, K
影响因子：
15.3
作者：
Bonecchi, R;Bianchi, G;Bordignon, P P;D'Ambrosio, D;Lang, R;Borsatti, A;Sozzani, S;Allavena, P;Gray, P A;Mantovani, A;Sinigaglia, F
通讯作者：
Sinigaglia, F
影响因子：
--
作者：
DANIELS, TE;WHITCHER, JP
通讯作者：
WHITCHER, JP