Mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats.
Mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats.
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蚊子寄生着对发育至关重要的细菌群落，但在当地栖息地之间差异很大。
DOI：
10.1111/mec.13877
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发表时间：
2016-11
影响因子：
4.9
通讯作者：
Strand MR
中科院分区：
文献类型：
--
作者：
Coon KL;Brown MR;Strand MR
蚊子是令人感兴趣的昆虫，因为几个物种将致病病原体传播给人类和其他脊椎动物。我们以前报道过，来自长期实验室培养的蚊子需要肠道中的活细菌才能发育，但发育并不依赖于特定种类的细菌。在这里，我们专注于三个不同但相互关联的研究领域，以更好地了解细菌在蚊子发育中的作用，通过研究来自美国东南部的埃及伊蚊，白纹伊蚊和致倦库蚊的野外和实验室种群。细菌16S rRNA基因扩增子的序列分析表明，细菌群落组成差异很大，在幼虫从不同的收集地点，而幼虫从同一网站共享的相似性。虽然以前不知道感染沃尔巴克氏体，结果也表明，Ae。来自一个实地地点的埃及人是双重感染的宿主。然而，无论收集地点或沃尔巴克氏体感染等因素如何，每种蚊子都需要消化道中的活细菌才能发育。结果还确定了使用抗生素消除幼虫中细菌群落的几个问题，以研究其发育后果。总而言之，我们的研究结果表明，几种蚊子的发育需要活的细菌。我们还假设这些物种并不依赖于特定的细菌，因为幼虫在它们定居的水生栖息地中不会遇到相同的细菌。
Mosquitoes are insects of interest because several species vector disease-causing pathogens to humans and other vertebrates. We previously reported that mosquitoes from long-term laboratory cultures require living bacteria in their gut to develop, but development does not depend on particular species of bacteria. Here, we focused on three distinct but interrelated areas of study to better understand the role of bacteria in mosquito development by studying field and laboratory populations of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus from the Southeastern United States. Sequence analysis of bacterial 16S rRNA gene amplicons showed that bacteria community composition differed substantially in larvae from different collection sites, whereas larvae from the same site shared similarities. Although previously unknown to be infected by Wolbachia, results also indicated that Ae. aegypti from one field site hosted a dual infection. Regardless of collection site or factors like Wolbachia infection, however, each mosquito species required living bacteria in their digestive tract to develop. Results also identified several concerns in using antibiotics to eliminate the bacterial community in larvae in order to study its developmental consequences. Altogether, our results indicate that several mosquito species require living bacteria for development. We also hypothesize these species do not rely on particular bacteria because larvae do not reliably encounter the same bacteria in the aquatic habitats they colonize.
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影响因子：
4.2
作者：
Duguma D;Hall MW;Rugman-Jones P;Stouthamer R;Terenius O;Neufeld JD;Walton WE
通讯作者：
Walton WE
影响因子：
4.2
作者：
Chouaia B;Rossi P;Epis S;Mosca M;Ricci I;Damiani C;Ulissi U;Crotti E;Daffonchio D;Bandi C;Favia G
通讯作者：
Favia G
影响因子：
4.6
作者：
Buck M;Nilsson LK;Brunius C;Dabiré RK;Hopkins R;Terenius O
通讯作者：
Terenius O
影响因子：
4.9
作者：
Anderson, Kirk E.;Russell, Jacob A.;Wheeler, Diana E.
通讯作者：
Wheeler, Diana E.
影响因子：
4.4
作者：
Baldo, Laura;Hotopp, Julie C. Dunning;Werren, John H.
通讯作者：
Werren, John H.