Optimization of Extraction or Purification Process of Multiple Components from Natural Products: Entropy Weight Method Combined with Plackett-Burman Design and Central Composite Design.
Optimization of Extraction or Purification Process of Multiple Components from Natural Products: Entropy Weight Method Combined with Plackett-Burman Design and Central Composite Design.
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熵权法结合Plackett-Burman设计和中心组合设计优化天然产物多组分提取或纯化工艺
DOI：
10.3390/molecules26185572
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发表时间：
2021-09-14
期刊：
影响因子：
--
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Du Y;Huang P;Jin W;Li C;Yang J;Wan H;He Y
本文采用熵权法结合Plackett-Burman设计和中心组合设计对多组分的提取纯化工艺进行了优化。以大孔树脂纯化黄芪皂苷为例，探讨了该方法的实用性。该方法首先利用EWM法确定各组分的权重，将各组分含量与其权重乘积之和作为综合得分，并以此作为评价指标。然后采用单因素法确定各因素的取值范围。应用PBD筛选显著性因素。通过CCD进一步优化重要变量，以确定最佳工艺参数。将EWM、PBD和CCD组合后，得到最佳纯化条件为：pH值6.0，提取溶剂浓度0.15 g/mL，乙醇体积分数75%。在最佳条件下，皂苷回收率的实际综合得分与预测值（n = 3）接近。本研究为天然产物中多种成分的提取纯化优化技术提供了一种简便、高效的方法。
In this paper, the optimization of the extraction/purification process of multiple components was performed by the entropy weight method (EWM) combined with Plackett–Burman design (PBD) and central composite design (CCD). We took the macroporous resin purification of Astragalus saponins as an example to discuss the practicability of this method. Firstly, the weight of each component was given by EWM and the sum of the product between the componential content and its weight was defined as the comprehensive score, which was taken as the evaluation index. Then, the single factor method was adopted for determining the value range of each factor. PBD was applied for screening the significant factors. Important variables were further optimized by CCD to determine the optimal process parameters. After the combination of EWM, PBD and CCD, the resulting optimal purification conditions were as follows: pH value of 6.0, the extraction solvent concentration of 0.15 g/mL, and the ethanol volume fraction of 75%. Under the optimal conditions, the practical comprehensive score of recoveries of saponins was close to the predicted value (n = 3). Therefore, the present study provided a convenient and efficient method for extraction and purification optimization technology of multiple components from natural products.
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