(1)H NMR-based metabolomics reveals the effect of maternal habitual dietary patterns on human amniotic fluid profile.
(1)H NMR-based metabolomics reveals the effect of maternal habitual dietary patterns on human amniotic fluid profile.
复制标题
DOI：
10.1038/s41598-018-22230-y
复制
发表时间：
2018-03-06
影响因子：
4.6
通讯作者：
Michaelidou AM
中科院分区：
文献类型：
--
作者：
Fotiou M;Fotakis C;Tsakoumaki F;Athanasiadou E;Kyrkou C;Dimitropoulou A;Tsiaka T;Chatziioannou AC;Sarafidis K;Menexes G;Theodoridis G;Biliaderis CG;Zoumpoulakis P;Athanasiadis AP;Michaelidou AM
母亲的饮食可能会影响后代的健康，即使在营养良好的人群中也是如此。羊水为胎儿代谢研究提供了一个合理的隔室。动物模型中的证据表明，母亲的饮食影响房颤的代谢特征；然而，来自人类研究的数据很少。因此，我们使用有效的食物频率调查问卷和实施基于核磁共振的代谢组学，探索了AF含量是否会受到母亲饮食的影响。对65例接受中期羊膜穿刺术产前诊断的妇女的房颤标本进行了分析。对补充的母体血清和尿液样本进行了分析。系统聚类分析确定了2种饮食模式，第1类(C1，n = 33)和第2类(C2，n = 32)。从精制谷类、黄色奶酪、红肉、家禽和“即食”食品中提取的能量比例显著较高，而从全谷类、蔬菜、水果、豆类和坚果中摄取的能量比例较高(P <； 0.05)。核磁共振氢谱可以确定与这些饮食模式相关的代谢物；葡萄糖、丙氨酸、酪氨酸、缬氨酸、柠檬酸、顺式乙酸酯和甲酸盐是C1AF样本中升高的关键鉴别代谢物。这是首次有证据表明房颤的构成受到母亲习惯饮食模式的影响。我们的研究结果强调了扩大孕期母体营养重要性知识的必要性。
Maternal diet may influence offspring’s health, even within well-nourished populations. Amniotic fluid (AF) provides a rational compartment for studies on fetal metabolism. Evidence in animal models indicates that maternal diet affects AF metabolic profile; however, data from human studies are scarce. Therefore, we have explored whether AF content may be influenced by maternal diet, using a validated food-frequency questionnaire and implementing NMR-based metabolomics. Sixty-five AF specimens, from women undergoing second-trimester amniocentesis for prenatal diagnosis, were analysed. Complementary, maternal serum and urine samples were profiled. Hierarchical cluster analysis identified 2 dietary patterns, cluster 1 (C1, n = 33) and cluster 2 (C2, n = 32). C1 was characterized by significantly higher percentages of energy derived from refined cereals, yellow cheese, red meat, poultry, and “ready-to-eat” foods, while C2 by higher (P < 0.05) whole cereals, vegetables, fruits, legumes, and nuts. 1H NMR spectra allowed the identification of metabolites associated with these dietary patterns; glucose, alanine, tyrosine, valine, citrate, cis-acotinate, and formate were the key discriminatory metabolites elevated in C1 AF specimens. This is the first evidence to suggest that the composition of AF is influenced by maternal habitual dietary patterns. Our results highlight the need to broaden the knowledge on the importance of maternal nutrition during pregnancy.
登录
查看更多内容
影响因子：
3.6
作者：
Bertram, Hanne Christine;Hoppe, Camilla;Michaelsen, Kim F.
通讯作者：
Michaelsen, Kim F.
母亲的饮食模式和妊娠结果
影响因子：
5.9
作者：
Chen X;Zhao D;Mao X;Xia Y;Baker PN;Zhang H
通讯作者：
Zhang H
影响因子：
6.2
作者：
Graca, Goncalo;Moreira, Ana Sofia;Gil, Ana M.
通讯作者：
Gil, Ana M.
影响因子：
3.6
作者：
Filntisi, Arianna;Fotakis, Charalambos;Cavouras, Dionisis
通讯作者：
Cavouras, Dionisis
影响因子：
3.9
作者：
Fotakis, C.;Zoga, M.;Zoumpoulakis, P.
通讯作者：
Zoumpoulakis, P.