Effect of silver, copper, mercury, and nickel ions on cellular proliferation during extended, low-dose exposures.
Effect of silver, copper, mercury, and nickel ions on cellular proliferation during extended, low-dose exposures.
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长期低剂量暴露期间银、铜、汞和镍离子对细胞增殖的影响。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
A. Schedle
中科院分区：
文献类型：
--
作者：
J. Wataha;P. Lockwood;A. Schedle
以前的研究已经证明并量化了金属离子在体外的细胞毒性，但这些报告的数据仅限于金属离子在细胞中的短期暴露（24-72小时）。然而，金属离子的长期、低剂量效应与牙科和其他生物医学合金的临床应用最为相关。因此，当前研究的目的是评估银、铜、汞和镍离子（已知从牙科合金中释放的四种金属离子）对单核细胞的长期影响。THP-1人单核细胞暴露于金属离子长达4周。金属离子的浓度是已知暴露24小时会引起细胞毒性的浓度的1-10%。每周检测细胞增殖和细胞活力。Ag（1+）和Hg（2+）对无活细胞的比例没有影响，但Cu（2+）和Ni（2+）随金属浓度的增加而增加了无活细胞的比例。这些影响仅对Ni（2+）在4周内累积。所有的金属离子都能显著减少细胞增殖，但每种金属离子的影响模式都是独特的，而且这种影响通常要到暴露3或4周后才会显现出来。目前的研究结果表明，从金属生物材料中释放的金属离子浓度低于先前报道的浓度可能会产生不利的生物效应。
Previous studies have demonstrated and quantified the cytotoxicity of metal ions in vitro, but the data from these reports have been limited to short-term exposures of metal ions to cells (24-72 h). Yet, the longer-term, low-dose effects of metal ions are most relevant to the clinical use of dental and other biomedical alloys. Thus, the purpose of the current study was to assess longer-term effects of ions of silver, copper, mercury, and nickel - four metal ions known to be released from dental alloys - on monocytes. THP-1 human monocytes were exposed to the metal ions for up to 4 weeks. Concentrations of the metal ions were 1-10% of those known to cause cytotoxicity with 24-h exposures. Cellular proliferation and cellular viability were measured weekly. Ag(1+) and Hg(2+) did not alter the percentage of nonviable cells, but Cu(2+) and Ni(2+) increased the nonviable component as a function of metal concentration. These effects were cumulative over the 4 weeks only for Ni(2+). All metal ions caused a significant reduction in cellular proliferation, but the pattern of the effect was unique to each metal ion, and the effects were often not evident until 3 or 4 weeks of exposure. The results of the current study indicate that metal ions released from metallic biomaterials may have adverse biological effects at concentrations lower than previously reported.
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DOI：
10.1002/jbm.820261004
发表时间：
1992
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
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DOI：
10.1016/0109-5641(94)90037-x
发表时间：
1994
期刊：
Dental materials : official publication of the Academy of Dental Materials
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作者：
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作者：
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DOI：
10.1161/01.atv.16.9.1095
发表时间：
1996
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Marczin,N;Antonov,A;Papapetropoulos,A;Munn,DH;Virmani,R;Kolodgie,FD;Gerrity,R;Catravas,JD
通讯作者：
Catravas,JD
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DOI：
10.1016/0109-5641(93)90080-a
发表时间：
1993
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
--
作者：
Bumgardner,JD;Lucas,LC;AlversonJr,MW;Tilden,AB
通讯作者：
Tilden,AB