Endogenous monoamines inhibit glutamate transmission in the spinal trigeminal nucleus of the guinea-pig.
Endogenous monoamines inhibit glutamate transmission in the spinal trigeminal nucleus of the guinea-pig.
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内源性单胺抑制豚鼠脊髓三叉神经核中的谷氨酸传输。
DOI：
10.1113/jphysiol.1996.sp021205
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Williams,JT
中科院分区：
文献类型：
--
作者：
Travagli,RA;Williams,JT
1.在豚鼠胶状质（SG）切片中使用全细胞膜片钳记录，我们研究了5-羟色胺（5-HT）和去甲肾上腺素（NA）介导的对初级传入刺激诱发的谷氨酸介导的EPSC的抑制。2. 5-HT和NA可降低自发性EPSP的频率。3. 5-HT引起的EPSC抑制由5-HT 1D受体亚型介导，因为5-HT 1D激动剂舒马曲坦（1 μ M）有效。4. NA和α 2-激动剂5-溴-N-（4，5-二氢-1H-咪唑-2-基）-6-喹喔啉胺（UK 14304）降低了EPSC，这种抑制作用被α 2-拮抗剂咪唑克生（1 μ M）和育亨宾（1 μ M）阻断。5. 5-HT-释放剂芬氟拉明（10 μ M）和Na-释放剂安非他明（1 μ M）也抑制EPSC。用5-HT耗竭剂对氯苯丙胺（10 μ M）预处理切片，减弱了芬氟拉明的抑制作用，但未能拮抗外源性5-HT的作用。6.这些体外结果表明，突触前抑制初级传入神经释放谷氨酸可以提供另一种机制来解释体内获得的5-HT和NA的抗伤害性作用。
1. With the use of whole‐cell patch clamp recordings in slices of guinea‐pig substantia gelatinosa (SG), we studied the serotonin (5‐HT)‐ and noradrenaline (NA)‐mediated inhibition of glutamate‐mediated EPSCs evoked from primary afferent stimulation. 2. The frequency of spontaneous EPSPs was reduced by 5‐HT and NA. 3. The inhibition of EPSCs caused by 5‐HT was mediated by the 5‐HT1D receptor subtype, since the 5‐HT1D agonist, sumatriptan (1 microM), was effective. 4. NA and the alpha 2‐agonist, 5‐bromo‐N‐(4,5‐dihydro‐1H‐imidazol‐2‐yl)‐6‐quinoxalinamine (UK 14304), decreased the EPSCs and this inhibition was blocked by the alpha 2‐antagonists, idazoxan (1 microM) and yohimbine (1 microM). 5. The 5‐HT‐releasing agent, fenfluramine (10 microM), and the Na‐releasing agent, amphetamine (1 microM), also depressed EPSCs. Pretreatment of slices with the 5‐HT‐depleting agent, p‐chloro‐amphetamine (10 microM), attenuated the inhibition of fenfluramine but failed to antagonize the effects of exogenously applied 5‐HT. 6. These in vitro results suggest that presynaptic inhibition of glutamate release from primary afferents can provide another mechanism to explain the antinociceptive effects of 5‐HT and NA obtained in vivo.
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影响因子：
7.3
作者：
CANFIELD, DR;DUNLAP, K
通讯作者：
DUNLAP, K
影响因子：
5.5
作者：
GRUDT, TJ;WILLIAMS, JT;TRAVAGLI, RA
通讯作者：
TRAVAGLI, RA
吗啡和去甲肾上腺素抑制大鼠脊髓切片中 P 物质的钾刺激释放，但 5-羟色胺和 γ-氨基丁酸不抑制
影响因子：
2.9
作者：
I. Pang;M. Vasko
通讯作者：
M. Vasko
影响因子：
2.5
作者：
TROMBLEY, PQ;WESTBROOK, GL
通讯作者：
WESTBROOK, GL
大鼠颌下腺和心房突触前α2-自身受体的药理学特征
影响因子：
7.3
作者：
N. Limberger;A. Trendelenburg;K. Starke
通讯作者：
K. Starke