Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine
Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine
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1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱修饰的氧化铁纳米粒子增强细胞摄取
DOI：
10.1039/c7ra06844a
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发表时间：
2017-07
期刊：
影响因子：
3.9
通讯作者：
Zhao Yingzheng
中科院分区：
文献类型：
--
作者：
Su Lichao;Zhang Baolin;Huang Yinping;Fan Ziliang;Zhao Yingzheng
纳米颗粒进入细胞以实现其生物学应用是重要的。我们合成并表征了用聚乙二醇（PEG）和聚乙烯亚胺（PEI）修饰的超顺磁性氧化铁纳米颗粒（SPIONs）（PEG/PEI-SPIONs），并进一步用1，2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）（DMPC-SPIONs）修饰以提高其细胞内摄取。通过透射电子显微镜（TEM）观察内化到PC-12细胞中的纳米颗粒，并通过电感耦合等离子体发射光谱（ICP-OES）进行定量。PC-12细胞对DMPC-SPION的摄取比PEG/PEI-SPION大得多，在溶酶体、内质网、线粒体、囊泡和细胞核周围发现了大量累积的纳米颗粒，一些纳米颗粒保留在细胞膜上。这可以归因于DMPC和膜磷脂的化学结构之间的相似性，这导致DMPC和细胞膜的融合。我们的研究结果鼓励DMPC-SPION作为药物载体，转染剂，细胞MRI造影剂和热疗剂的进一步研究。
It is important for nanoparticles to enter cells to implement their biological applications. We synthesized and characterized superparamagnetic iron oxide nanoparticles (SPIONs) modified with poly(ethylene glycol) (PEG) and polyethyleneimine (PEI) (PEG/PEI-SPIONs), and further with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) (DMPC-SPIONs) to improve their intracellular uptake. The nanoparticles internalized into PC-12 cells were observed by transmission electron microscopy (TEM) and quantified by inductively coupled plasma emission spectroscopy (ICP-OES). There was a much greater uptake of DMPC-SPIONs than PEG/PEI-SPIONs into PC-12 cells and remarkable amounts of accumulated nanoparticles were found in the lysosome, endoplasmic reticulum, mitochondria, vesicles, and around the nucleus, and some nanoparticles remained on the cell membrane. This can be attributed to the similarity between the chemical structures of DMPC and membrane phospholipids which result in the fusion of DMPC and cell membranes. Our results encourage further research on DMPC-SPIONs as drug carriers, transfection agents, MRI contrast agents for cells and hyperthermia agents.
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