Sex steroid receptor expression and localization in benign prostatic hyperplasia varies with tissue compartment.
Sex steroid receptor expression and localization in benign prostatic hyperplasia varies with tissue compartment.
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DOI：
10.1016/j.diff.2013.02.006
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发表时间：
2013-04
期刊：
影响因子：
2.9
通讯作者：
Ricke, William A.
中科院分区：
文献类型：
--
作者：
Nicholson, Tristan M.;Sehgal, Priyanka D.;Drew, Sally A.;Huang, Wei;Ricke, William A.
雄激素和雌激素通过它们各自的受体起作用，在良性前列腺增生（BPH）中起重要作用。本研究的目的是定量表征雄激素受体（AR）和雌激素受体α (ERα)的组织分布和染色强度，并评估人类前列腺增生与正常前列腺中表达AR和ERα的细胞。采用正常前列腺和前列腺增生组织组成的组织微阵列，采用多重免疫组化检测AR和ERα。我们使用多光谱成像平台进行自动扫描，组织和细胞分割和标记量化。BPH标本上皮细胞和间质细胞数量增加，上皮细胞百分比增加。在间质和上皮中，前列腺增生的平均核面积相对于正常前列腺减少。与正常前列腺相比，前列腺增生组织上皮细胞和基质细胞中AR的表达和染色强度显著增加。ERα在BPH上皮中表达升高。然而，与正常前列腺相比，BPH的间质ERα表达和染色强度降低。双阳性（AR和ERα）上皮细胞在BPH中多见，双阴性（AR和ERα）间质细胞和上皮细胞阴性细胞在BPH中较少见。这些数据强调了前列腺组织层定位和类固醇激素受体表达的重要性。了解雄激素和雌激素的组织特异性激素作用将有助于更好地了解前列腺发病机制，并可能有助于更好地治疗前列腺增生。
Androgens and estrogens, acting via their respective receptors, are important in benign prostatic hyperplasia (BPH). The goal of this study was to quantitatively characterize the tissue distribution and staining intensity of androgen receptor (AR) and estrogen receptor-alpha (ERα), and assess cells expressing both AR and ERα, in human BPH compared to normal prostate. A tissue microarray composed of normal prostate and BPH tissue was used and multiplexed immunohistochemistry was performed to detect AR and ERα. We used a multispectral imaging platform for automated scanning, tissue and cell segmentation and marker quantification. BPH specimens had an increased number of epithelial and stromal cells and increased percentage of epithelium. In both stroma and epithelium, the mean nuclear area was decreased in BPH relative to normal prostate. AR expression and staining intensity in epithelial and stromal cells was significantly increased in BPH compared to normal prostate. ERα expression was increased in BPH epithelium. However, stromal ERα expression and staining intensity was decreased in BPH compared to normal prostate. Double positive (AR & ERα) epithelial cells were more prevalent in BPH, and fewer double negative (AR & ERα) stromal and epithelial negative cells were observed in BPH. These data underscore the importance of tissue layer localization and expression of steroid hormone receptors in the prostate. Understanding the tissue-specific hormone action of androgens and estrogens will lead to a better understanding of mechanisms of pathogenesis in the prostate and may lead to better treatment for BPH.
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影响因子：
4.8
作者：
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