Pulsed magneto-motive ultrasound imaging to detect intracellular trafficking of magnetic nanoparticles.
Pulsed magneto-motive ultrasound imaging to detect intracellular trafficking of magnetic nanoparticles.
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DOI：
10.1088/0957-4484/22/41/415105
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发表时间：
2011-10-14
期刊：
影响因子：
3.5
通讯作者：
Emelianov SY
中科院分区：
文献类型：
--
作者：
Mehrmohammadi M;Qu M;Ma LL;Romanovicz DK;Johnston KP;Sokolov KV;Emelianov SY
随着纳米粒子在医学成像和生物医学中的应用迅速扩大，纳米粒子与活细胞的相互作用已成为一个积极关注的领域。例如，由于纳米颗粒聚集体的光学性质的变化，已经使用等离子体纳米颗粒和光学或基于光学的技术很好地研究了纳米颗粒的细胞内运输-细胞-纳米颗粒相互作用的重要部分。然而，磁性纳米颗粒，尽管其广泛的临床应用，不表现出等离子体共振特性，因此它们的细胞内聚集不能检测到基于光学的成像技术。在这项研究中，我们研究了一种新的成像技术的可行性-脉冲磁动超声（pMMUS），以确定内吞磁性纳米颗粒的细胞内运输。在脉冲磁动超声成像中，使用聚焦的高强度脉冲磁场来激发标记有磁性纳米颗粒的细胞，然后使用超声成像来监测组织的机械响应。我们先前证明，与来自单个纳米颗粒的信号相比，磁性纳米颗粒的簇放大了pMMUS信号。在这里，我们进一步证明pMMUS成像可以识别磁性纳米颗粒和活细胞之间的相互作用，即细胞内纳米颗粒的细胞内聚集。我们的研究结果表明，pMMUS成像不仅可以检测磁性纳米颗粒的存在，而且还可以非侵入性地实时提供有关其细胞内运输的信息。
As applications of nanoparticles in medical imaging and biomedicine rapidly expand, the interactions of nanoparticles with living cells have become an area of active interest. For example, intracellular trafficking of nanoparticles – an important part of cell-nanoparticle interaction, has been well studied using plasmonic nanoparticles and optical or optics-based techniques due to the change in optical properties of the nanoparticle aggregates. However, magnetic nanoparticles, despite their wide range of clinical applications, do not exhibit plasmonic-resonant properties and therefore their intracellular aggregation cannot be detected by optics-based imaging techniques. In this study, we investigated the feasibility of a novel imaging technique – pulsed magneto-motive ultrasound (pMMUS), to identify intracellular trafficking of endocytosed magnetic nanoparticles. In pulsed magneto-motive ultrasound imaging a focused, high intensity, pulsed magnetic field is used to excite the cells labeled with magnetic nanoparticles, and ultrasound imaging is then used to monitor the mechanical response of the tissue. We demonstrated previously that clusters of magnetic nanoparticles amplify the pMMUS signal in comparison to signal from individual nanoparticles. Here we further demonstrate that pMMUS imaging can identify interaction between magnetic nanoparticles and living cells, i.e. intracellular aggregation of nanoparticles within the cells. The results of our study suggest that pMMUS imaging can not only detect the presence of magnetic nanoparticles but also provides information about their intracellular trafficking non-invasively and in real-time.
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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