Small multifunctional nanoclusters (nanoroses) for targeted cellular imaging and therapy.
Small multifunctional nanoclusters (nanoroses) for targeted cellular imaging and therapy.
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DOI：
10.1021/nn900440e
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发表时间：
2009-09-22
期刊：
影响因子：
17.1
通讯作者：
Johnston KP
中科院分区：
文献类型：
--
作者：
Ma LL;Feldman MD;Tam JM;Paranjape AS;Cheruku KK;Larson TA;Tam JO;Ingram DR;Paramita V;Villard JW;Jenkins JT;Wang T;Clarke GD;Asmis R;Sokolov K;Chandrasekar B;Milner TE;Johnston KP
20-50纳米颗粒靶向和调节特定类型细胞生物学的能力将使癌症和动脉粥样硬化的细胞成像和治疗取得重大进展。一个关键的挑战是将高度的靶向、成像和治疗功能加载到小而稳定的颗粒中。本文报道了通过动力学控制自组装形成的约30 nm稳定均匀尺寸的近红外（NIR）活性超顺磁性纳米团簇。纳米复合粒子的受控组装成具有小主粒子间距的簇，产生电子的集体响应，将吸光度转移到近红外区域。由~70氧化铁初级颗粒组成的纳米团簇具有较薄的金涂层，具有较强的近红外（700 ~ 850 nm）吸光度，截面为~10 ~ 14 m2。由于平均厚度仅为2nm的薄金壳，r2自旋磁弛豫为219 mM−1 s−1，比具有较厚金壳的典型氧化铁粒子的观测值大一个数量级。尽管聚合物稳定剂的质量比只有12%，但在去离子水中，8个月以上的粒径和近红外吸光度变化很小。葡聚糖包被促进了巨噬细胞对纳米团簇的高度摄取，在细胞培养和体内动脉粥样硬化兔模型中，在暗场和高光谱显微镜下产生强烈的近红外对比。通过纳米颗粒构建块的组装而设计的具有光学、磁性和治疗功能的小纳米团簇，为靶向细胞成像、治疗以及联合成像和治疗提供了广泛的机会。
The ability of 20–50 nm nanoparticles to target and modulate the biology of specific types of cells will enable major advancements in cellular imaging and therapy in cancer and atherosclerosis. A key challenge is to load an extremely high degree of targeting, imaging, and therapeutic functionality into small, yet stable particles. Herein we report ~30 nm stable uniformly sized near-infrared (NIR) active, superparamagnetic nanoclusters formed by kinetically controlled self-assembly of gold-coated iron oxide nanoparticles. The controlled assembly of nanocomposite particles into clusters with small primary particle spacings produces collective responses of the electrons that shift the absorbance into the NIR region. The nanoclusters of ~70 iron oxide primary particles with thin gold coatings display intense NIR (700–850 nm) absorbance with a cross section of ~10−14 m2. Because of the thin gold shells with an average thickness of only 2 nm, the r2 spin–spin magnetic relaxivity is 219 mM−1 s−1, an order of magnitude larger than observed for typical iron oxide particles with thicker gold shells. Despite only 12% by weight polymeric stabilizer, the particle size and NIR absorbance change very little in deionized water over 8 months. High uptake of the nanoclusters by macrophages is facilitated by the dextran coating, producing intense NIR contrast in dark field and hyperspectral microscopy, both in cell culture and an in vivo rabbit model of atherosclerosis. Small nanoclusters with optical, magnetic, and therapeutic functionality, designed by assembly of nanoparticle building blocks, offer broad opportunities for targeted cellular imaging, therapy, and combined imaging and therapy.
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影响因子：
3.5
作者：
Larson, Timothy A.;Bankson, James;Sokolov, Konstantin
通讯作者：
Sokolov, Konstantin
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
56.9
作者：
DeVries, Gretchen A.;Brunnbauer, Markus;Stellacci, Francesco
通讯作者：
Stellacci, Francesco
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
82.9
作者：
Lee, Jae-Hyun;Huh, Yong-Min;Cheon, Jinwoo
通讯作者：
Cheon, Jinwoo